| Field | Specification |
|---|---|
| Mfr No | |
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| Conjugate | |
| Host | |
| Product Type | |
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| UniProt # |
Product Overview
Mast cell tryptase(MCT) is widely expressed in respiratory tract, gastrointestinal tract, skin and other tissues. It is mainly used in the study of respiratory system, gastrointestinal system diseases and their tumors.
Validated Applications & Recommended Dilutions
| Application | Recommended Dilution |
|---|---|
| Immunohistochemistry | See product datasheet |
Antibody Specifications
| Target | the target protein |
|---|---|
| Host Species | Mouse |
| Clonality | Monoclonal |
| Isotype | |
| Conjugate | Unconjugated |
| Clone Number | YN01036m |
| Purification | See datasheet |
| Concentration |
Immunogen
Recombinant protein fragment; see full datasheet. UniProt: Q15661.
Species Reactivity & Cross-Reactivity
Validated for: Human. Verify suitability in your sample type prior to use. For species or applications not listed in the datasheet, consult our technical team before purchase.
Storage & Handling
The RTU should be stored at 2~8°C protecting from light (Do not freeze),Valid for 12 months.
Storage Buffer:
Related Products
Browse additional antibodies targeting the target protein and complementary reagents — including secondary antibodies, blocking buffers, and detection kits — available through BioHippo.
For IHC-P, heat-induced epitope retrieval (HIER) is typically required. Recommended starting conditions: citrate buffer pH 6.0 (microwave or pressure cooker, ~20 min) for rodent samples; EDTA buffer pH 9.0 for human clinical samples. After retrieval, allow sections to cool gradually before proceeding to blocking. Include a the target-positive tissue control (confirmed by literature or database expression data) and a negative isotype control in every run. Recommended dilution: see datasheet. Refer to the product datasheet for any target-specific retrieval guidance.
This antibody has been experimentally validated in: Human. Cross-reactivity with other species has not been systematically evaluated. If your sample species is not listed, assess immunogen sequence homology to your target species ortholog. The immunogen used is: "" — higher sequence identity between species increases the probability of cross-reactivity, but experimental validation with appropriate controls is necessary before drawing scientific conclusions.
The RTU should be stored at 2~8°C protecting from light (Do not freeze),Valid for 12 months. To maintain antibody activity, avoid repeated freeze-thaw cycles. Aliquot into single-use volumes before first freeze. If working with the antibody frequently, keep a working stock at 4°C for up to 4 weeks — add 0.02% sodium azide to the working stock as a bacteriostatic agent if azide is not already present in the buffer. Monitor performance against a validated positive control when transitioning between storage aliquots or lots.
This monoclonal antibody (clone YN01036m) provides highly consistent, epitope-specific recognition of the target, making it ideal for quantitative assays, multiplex panels, and studies requiring strict reproducibility across experiments and independent laboratories. Unlike polyclonal preparations, each production batch from a single hybridoma clone generates antibody with identical epitope specificity, affinity, and isotype — ensuring reliable signal comparison in longitudinal studies or multi-site collaborations. When citing this antibody in publications, include the clone designation (clone YN01036m) to enable third-party reproducibility verification.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
