| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MECP2 recombinant protein (Position: K36-Q437) was used as the immunogen for the MECP2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MECP2 Antibody / Methyl-CpG-binding protein 2 is a anti-MECP2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Monkey, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: MECP2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ELISA
Biological background
Functionally, MECP2 antibody identifies a 486-amino-acid protein composed of a methyl-CpG-binding domain (MBD) and a transcriptional repression domain (TRD). MECP2 binds selectively to methylated DNA sequences and recruits co-repressors such as SIN3A and histone deacetylases (HDACs), leading to histone deacetylation and chromatin condensation. It also regulates non-coding RNA transcription, alternative splicing, and higher-order chromatin organization. MECP2 plays a critical role in neuronal maturation, synaptic development, and activity-dependent gene regulation.
The MECP2 gene is located on chromosome Xq28 and is expressed ubiquitously, but at especially high levels in neurons. Expression is dynamically regulated during postnatal brain development, paralleling synaptogenesis. In neurons, MECP2 controls transcriptional homeostasis by balancing activation and repression of large gene networks essential for synaptic plasticity and neuronal connectivity.
Pathologically, mutations in MECP2 cause Rett syndrome, a severe neurodevelopmental disorder characterized by regression of motor and cognitive abilities in early childhood. Different mutation types (missense, nonsense, deletions) disrupt DNA binding or repressor recruitment, leading to widespread transcriptional dysregulation. Altered MECP2 expression is also implicated in autism spectrum disorder and certain cancers. Research using MECP2 antibody supports studies in epigenetic regulation, neurodevelopment, and chromatin biology.
MECP2 antibody is validated for western blotting, immunofluorescence, and chromatin immunoprecipitation to detect methyl-DNA-binding proteins.
Structurally, Methyl-CpG-binding protein 2 contains an MBD that recognizes methylated CpG dinucleotides, a TRD that interacts with SIN3A-HDAC complexes, and a C-terminal domain that binds nucleosomes. This structural modularity allows MECP2 to act as a chromatin architectural protein coordinating DNA methylation and transcriptional silencing. This antibody enables detailed analysis of MECP2's role in gene regulation and neurological disease mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
