| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MED28 recombinant protein (Position: M1-T178) was used as the immunogen for the MED28 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MED28 Antibody / Mediator complex subunit 28 is a anti-MED28 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunoprecipitation (IP), Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: MED28
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IP, WB
Biological background
MED28 is encoded by the MED28 gene located on human chromosome 4q27. The protein is approximately 178 amino acids long and localizes to both the nucleus and plasma membrane. MED28 interacts with other Mediator complex subunits as well as cytoskeletal proteins, suggesting dual functions in transcriptional control and cell structure regulation. Its nuclear role involves recruitment of RNA polymerase II to promoters and modulation of gene-specific transcriptional activation.
The MED28 antibody detects a 22 kilodalton band by western blot and shows nuclear and cortical cytoplasmic staining. MED28 acts as a scaffold linking the cytoskeleton to transcriptional machinery, coordinating extracellular signals with gene expression. It associates with receptor tyrosine kinases and small GTPases, influencing actin organization and cell migration.
Functionally, MED28 contributes to development and differentiation by regulating transcription factors involved in lineage commitment. Dysregulation of MED28 expression has been linked to various cancers, including breast and gastric carcinoma, where it enhances proliferation and motility. MED28 also participates in smooth muscle differentiation and cardiovascular development, underscoring its diverse physiological roles.
Through its integration of signaling and transcriptional regulation, MED28 serves as a nexus between cellular structure and gene expression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
