| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MED4 recombinant protein (Position: R27-H247) was used as the immunogen for the MED4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MED4 Antibody / Mediator complex subunit 4 is a anti-MED4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: MED4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
MED4 antibody detects a protein that resides in the nucleus and is widely expressed across tissues, with particularly high expression in actively proliferating cells. MED4 functions as a structural component that helps assemble and maintain the integrity of the Mediator complex. The protein interacts with other Mediator subunits, including MED6, MED7, and MED8, to promote preinitiation complex formation and transcriptional activation. Through its participation in this multi-subunit assembly, MED4 indirectly influences processes such as cell cycle regulation, metabolic control, and differentiation.
Functionally, MED4 acts as a coregulator for a variety of signaling pathways, including those mediated by nuclear hormone receptors, Myc, and p53. The Mediator complex serves as a bridge between enhancer-bound transcription factors and the basal transcriptional machinery, making MED4 indispensable for transcriptional initiation and elongation. Disruption of MED4 or other Mediator components can impair RNA synthesis and contribute to developmental defects or disease. Recent studies suggest that the Mediator complex, including MED4, may participate in transcriptional pausing and gene-specific regulation in response to environmental and stress cues.
Mutations or copy number variations affecting the MED4 gene have been implicated in neurodevelopmental disorders and congenital anomalies, underscoring its role in transcriptional control during embryogenesis. Aberrant expression of MED4 has also been observed in various cancers, where altered Mediator activity promotes oncogenic transcriptional programs. Functional proteomics analyses show that MED4 participates in chromatin looping and enhancer-promoter communication, critical for high-fidelity transcriptional activation. Structurally, MED4's helical regions allow it to form coiled-coil interactions within the Mediator head domain, ensuring complex stability.
In disease contexts, dysregulated Mediator signaling has been associated with metabolic syndromes, inflammatory diseases, and tumor progression. MED4 expression correlates with proliferative gene signatures in certain cancers, suggesting its potential role as a biomarker of active transcriptional states. Because of its conserved function, MED4 serves as an essential reference subunit for Mediator complex studies across species.
Immunohistochemical staining using MED4 antibody demonstrates nuclear localization in epithelial and neuronal tissues, consistent with its function in transcriptional regulation. MED4 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
