| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MEIOB recombinant protein (Position: I140-V442) was used as the immunogen for the MEIOB antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MEIOB Antibody / Meiosis-specific with OB domains protein is a anti-MEIOB Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MEIOB
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Functionally, MEIOB antibody identifies a 471-amino-acid protein that localizes to meiotic chromatin in germ cells. MEIOB interacts with single-stranded DNA at resected DSBs and forms a complex with SPATA22, which stabilizes the DNA repair intermediate and promotes strand exchange facilitated by recombinases DMC1 and RAD51. Together, these interactions drive the homologous recombination events that generate genetic diversity during gametogenesis.
The MEIOB gene is located on chromosome 16p13.3 and is expressed exclusively in meiotic cells of the testis and ovary. MEIOB contains oligonucleotide/oligosaccharide-binding (OB) folds characteristic of single-stranded DNA-binding proteins. Its expression begins at the leptotene stage of meiosis and persists through pachytene, where it coordinates crossover formation and synapsis of homologous chromosomes. Loss of MEIOB function results in defective recombination, impaired synaptonemal complex formation, and meiotic arrest.
In mammals, MEIOB plays an indispensable role in meiotic DSB repair by stabilizing single-stranded DNA intermediates and promoting strand invasion. It also prevents excessive nuclease activity that could lead to genome instability. In humans, MEIOB mutations have been linked to infertility due to defective gametogenesis, emphasizing its essential contribution to reproductive success. In addition to its germline function, MEIOB expression may serve as a biomarker for spermatogenic activity and ovarian follicle development.
MEIOB antibody is widely used in reproductive biology, genetics, and DNA repair research. It is suitable for immunohistochemistry, immunoblotting, and chromatin association studies to track MEIOB localization and complex formation. This antibody supports investigations into meiotic recombination, chromosomal synapsis, and genetic stability. It is also used in infertility research to assess meiotic defects and germ cell differentiation.
Structurally, MEIOB possesses two OB-fold domains for DNA binding and forms a stoichiometric complex with SPATA22 that mimics the RPA-like architecture.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
