| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | E.coli-derived human MFI2/MELTF recombinant protein (Position: A60-D647) was used as the immunogen for the MELTF antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MELTF Antibody / Melanotransferrin / MFI2 is a anti-MELTF Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MELTF
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
MELTF antibody targets a glycosylphosphatidylinositol (GPI)-anchored membrane protein of approximately 80 kDa that facilitates cellular iron uptake independent of the classical transferrin receptor pathway. It is expressed in the placenta, intestine, and brain capillaries, where it contributes to iron delivery across epithelial and endothelial barriers. In cancer biology, MELTF is overexpressed in melanomas, glioblastomas, and certain epithelial tumors, where it supports tumor cell proliferation and survival under low-iron conditions. Elevated MELTF expression is associated with increased metastatic potential and angiogenesis through modulation of iron availability.
The MELTF antibody is useful in identifying tumor-associated expression and studying iron metabolism mechanisms distinct from transferrin receptor 1 (TFRC) or 2 (TFR2). Structurally, MELTF shares domain homology with transferrins, including two lobes capable of binding ferric iron, but differs by being GPI-anchored rather than secreted. The MELTF gene is located on chromosome 3q13.31 and encodes a 738-amino acid protein that undergoes glycosylation and cleavage to form the mature membrane-associated form. Its regulation is influenced by iron status, hypoxia-inducible factors (HIFs), and oncogenic signaling pathways such as MAPK and PI3K/AKT.
Recent studies indicate MELTF may function in neuroprotection by mediating iron uptake in the blood-brain barrier, and mutations or autoantibodies to MELTF have been linked to neurodegenerative disorders. MELTF antibody detection in immunohistochemistry and flow cytometry enables quantification of cell-surface expression, while western blotting reveals glycosylated isoforms. MELTF also plays roles in embryonic development, wound healing, and immune modulation by regulating local iron concentrations.
As an iron-binding antigen with diagnostic and therapeutic potential, MELTF antibody supports research in oncology, neurobiology, and iron transport regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
