| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MEP1B recombinant protein (Position: H73-Q489) was used as the immunogen for the MEP1B antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MEP1B Antibody / Meprin A subunit beta is a anti-MEP1B Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MEP1B
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ELISA
Biological background
Meprin A subunit beta is synthesized as a zymogen and activated through proteolytic cleavage. Once active, it catalyzes the hydrolysis of various substrates, including extracellular matrix proteins such as collagen, fibronectin, and laminin, as well as cytokines and growth factors. These activities make Meprin A subunit beta a crucial regulator of tissue remodeling and inflammatory signaling. The MEP1B antibody allows scientists to detect this enzyme in epithelial cells, intestinal mucosa, and kidney proximal tubules, where it plays essential roles in maintaining barrier function and proteolytic balance.
Mutations or altered expression of MEP1B have been linked to inflammatory bowel disease, fibrosis, and cancer. Overexpression may enhance degradation of matrix components, facilitating tumor invasion or chronic tissue injury, while loss of function can impair epithelial integrity. The MEP1B antibody enables evaluation of these changes in expression patterns, helping clarify the connection between Meprin A subunit beta and disease pathology. Recent studies also suggest Meprin B's involvement in activation of interleukin precursors and complement components, further extending its biological significance.
At the molecular level, Meprin A subunit beta anchors to the plasma membrane via a transmembrane domain, orienting its catalytic site extracellularly. It interacts with glycoproteins and proteoglycans that influence its enzymatic specificity and activity. The MEP1B antibody is an effective tool for studying these protein�protein interactions using western blotting, immunohistochemistry, and immunofluorescence. Researchers frequently apply it in tissue-based assays to evaluate distribution and regulation in kidney, intestine, and liver, where the enzyme's expression is most prominent.
Beyond normal physiology, Meprin A subunit beta has drawn attention for its potential contribution to neurodegenerative and fibrotic disorders. Proteolytic imbalance caused by excess Meprin activity can exacerbate inflammatory cascades and extracellular matrix turnover. The MEP1B antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
