| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MEPCE recombinant protein (Position: G79-L651) was used as the immunogen for the MEPCE antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MEPCE Antibody / 7SK snRNA methylphosphate capping enzyme is a anti-MEPCE Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: MEPCE
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Functionally, MEPCE antibody identifies a 689-amino-acid enzyme that binds to 7SK snRNA within the 7SK ribonucleoprotein complex. MEPCE cooperates with LARP7 and HEXIM1 to maintain repression of positive transcription elongation factor b (P-TEFb), thereby controlling RNA polymerase II elongation and global transcriptional output. It serves as a key regulator of gene expression homeostasis.
The MEPCE gene is located on chromosome 7q22.3 and is ubiquitously expressed, with enrichment in transcriptionally active tissues such as brain, liver, and gonads. MEPCE function is essential for normal development and maintenance of chromatin transcription dynamics.
Pathologically, mutations or reduced expression of MEPCE cause neurodevelopmental disorders characterized by intellectual disability and seizures. Disruption of 7SK complex stability leads to uncontrolled transcription elongation and altered gene expression. Research using MEPCE antibody supports studies in RNA biology, transcription regulation, and neurological disease mechanisms.
MEPCE antibody is validated for western blotting, immunoprecipitation, and immunofluorescence to detect RNA methyltransferases and transcriptional regulators.
Structurally, 7SK snRNA methylphosphate capping enzyme contains a conserved methyltransferase domain that binds S-adenosylmethionine and the 7SK RNA substrate. This antibody facilitates investigation of MEPCE's enzymatic role in transcriptional repression and RNA stability.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
