| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MEST recombinant protein (Position: A21-D320) was used as the immunogen for the MEST antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MEST Antibody / Mesoderm-specific transcript protein is a anti-MEST Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MEST
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
Functionally, MEST antibody identifies a 335-amino-acid protein that contains a predicted hydrolase fold and is implicated in adipocyte differentiation, embryonic growth, and metabolic regulation. Although its enzymatic substrate remains uncertain, structural homology suggests esterase or lipase-like activity. MEST is expressed during mesoderm formation and plays a key role in development of the placenta, skeletal muscle, and adipose tissue. Elevated MEST expression correlates with obesity, insulin resistance, and altered lipid storage, suggesting a regulatory role in adipogenesis and energy metabolism.
The MEST antibody is used to study genomic imprinting, epigenetic regulation, and developmental gene expression. MEST is a key marker for parent-of-origin gene expression studies, given that it is expressed exclusively from the paternal allele in most tissues. The MEST gene is located on chromosome 7q32.2 and lies within an imprinted domain regulated by differential DNA methylation. Aberrant methylation or loss of imprinting at this locus has been associated with developmental disorders such as Silver-Russell syndrome and growth abnormalities.
In adult tissues, MEST expression is detected in adipocytes, brain, and pancreas, where it influences energy balance and metabolic plasticity. Research suggests that MEST interacts with Wnt signaling pathways to regulate cell fate determination and tissue remodeling. In cancer, MEST dysregulation has been linked to metastasis and epithelial-to-mesenchymal transition (EMT). Overexpression of MEST promotes cell migration and invasion, while silencing reduces tumor aggressiveness.
MEST antibody applications include western blotting, immunofluorescence, and RT-qPCR normalization in imprinting and developmental biology research. Because of its epigenetic regulation, MEST serves as a valuable biomarker for studying parental imprinting effects and obesity-related gene expression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
