| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human METTL17 recombinant protein (Position: K129-R429) was used as the immunogen for the METTL17 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
METTL17 Antibody / Methyltransferase-like protein 17 is a anti-METTL17 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human. Reported localization: Nuclear.
Key elements and design rationale
- Target: METTL17
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, ELISA
Biological background
METTL17 is encoded by the METTL17 gene located on human chromosome 11q24.2. The protein localizes primarily to the mitochondrial matrix, where it associates with mitochondrial ribosomal subunits. Structurally, METTL17 contains a conserved Rossmann-like fold typical of class I methyltransferases, which facilitates binding to S-adenosyl-L-methionine (SAM), the universal methyl donor. Loss of METTL17 function impairs mitochondrial translation, leading to reduced oxidative phosphorylation and energy production.
The METTL17 antibody is useful in identifying both endogenous and overexpressed protein in cell lysates. Western blot typically reveals a single band around 44�46 kDa corresponding to full-length METTL17. Immunofluorescence analysis demonstrates mitochondrial localization with co-staining of mitochondrial markers such as TOM20 or COX IV. Functional studies have shown that METTL17 interacts with mitochondrial ribosomal RNA and components of the large ribosomal subunit, supporting its role in rRNA modification and mitoribosome assembly.
Defects in METTL17-mediated methylation have been linked to disrupted mitochondrial respiration and altered cellular energy metabolism. In addition to its mitochondrial functions, METTL17 expression has been observed in proliferating cells and certain cancer tissues, suggesting potential links between RNA methylation and tumor metabolism.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
