| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MFAP3L recombinant protein (Position: K29-V409) was used as the immunogen for the MFAP3L antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MFAP3L Antibody / Microfibril-associated protein 3-like is a anti-MFAP3L Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MFAP3L
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, ELISA
Biological background
Functionally, MFAP3L antibody identifies a 386-amino-acid cytoplasmic protein that associates with signaling molecules such as small GTPases, actin-binding proteins, and scaffolding complexes. MFAP3L contributes to cytoskeletal organization and may facilitate communication between extracellular matrix components and intracellular signaling pathways. Its expression has been linked to the regulation of cell migration, proliferation, and differentiation.
The MFAP3L gene is located on chromosome 4q32.1 and encodes a protein expressed in diverse tissues, including heart, lung, and skeletal muscle. MFAP3L shares sequence similarity with other microfibril-associated proteins but lacks classical extracellular matrix domains, suggesting a distinct intracellular regulatory role. It has been proposed to interact with Rho family GTPases and participate in actin cytoskeleton remodeling during cell movement and adhesion.
In cancer biology, elevated MFAP3L expression has been observed in certain epithelial tumors, including breast and colorectal cancer, where it may contribute to increased motility and invasive potential. Conversely, in cardiac and skeletal muscle, MFAP3L may assist in maintaining structural stability under mechanical stress. Although its specific mechanisms remain to be fully elucidated, MFAP3L appears to function at the interface of cytoskeletal dynamics and signal transduction.
MFAP3L antibody is widely used in cell biology, oncology, and signal transduction research. It is suitable for immunoblotting, immunofluorescence, and co-immunoprecipitation to study MFAP3L localization and binding partners. This antibody supports research into cytoskeletal control, extracellular matrix signaling, and tumor progression. In developmental studies, MFAP3L serves as a marker of cytoplasmic microfibril-associated proteins involved in tissue structure and mechanical adaptation.
Structurally, MFAP3L contains multiple coiled-coil regions that promote protein complex formation and interaction with cytoskeletal regulators. Its expression may be modulated by growth factor signaling and mechanical cues.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
