| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MFSD2A recombinant protein (Position: Q22-L543) was used as the immunogen for the MFSD2A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MFSD2A Antibody / Major facilitator superfamily domain-containing protein 2A is a anti-MFSD2A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Punctate cytoplasmic, ER, cell membrane.
Key elements and design rationale
- Target: MFSD2A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, ELISA
Biological background
MFSD2A is encoded by the MFSD2A gene located on human chromosome 1p34.2. The protein belongs to the major facilitator superfamily of solute carriers and is approximately 530 amino acids in length, with 12 predicted transmembrane helices. MFSD2A localizes to the plasma membrane of endothelial cells lining brain capillaries, where it functions as a transporter for lysophosphatidylcholine-bound polyunsaturated fatty acids. Unlike most nutrient transporters at the BBB, MFSD2A is not energy-dependent but couples lipid import to sodium gradients.
The MFSD2A antibody detects a 62 kilodalton band in western blot and demonstrates strong vascular endothelial staining in brain tissue and retina. In mouse models, deletion of MFSD2A results in severe microcephaly, neuronal loss, and impaired synaptic development due to reduced DHA delivery to the brain. In adults, MFSD2A maintains BBB tightness by suppressing vesicular transcytosis, ensuring selective permeability and protecting the central nervous system from toxins and immune cell infiltration.
MFSD2A expression is regulated by pericytes and Wnt/beta-catenin signaling pathways, linking vascular maturation to lipid metabolism. Its dysfunction has been associated with neurodevelopmental disorders, cognitive impairment, and visual defects. In cancer research, MFSD2A acts as a metastasis suppressor by inhibiting vascular leakage and tumor cell extravasation, demonstrating a dual role in nutrient transport and vascular homeostasis.
Beyond the nervous system, MFSD2A participates in lipid absorption in the placenta and peripheral vasculature. Because of its essential function in brain DHA uptake, MFSD2A is a promising target for therapeutic strategies aimed at enhancing neuroprotection and treating BBB disorders.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
