| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MFSD4A recombinant protein (Position: S48-R514) was used as the immunogen for the MFSD4A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MFSD4A Antibody / Major facilitator superfamily domain-containing protein 4A is a anti-MFSD4A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: MFSD4A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
MFSD4A antibody identifies a multi-pass transmembrane protein that contains 12 predicted alpha-helical transmembrane domains arranged in a canonical MFS fold. This structure allows alternating access transport of solutes driven by proton or sodium gradients. The cytoplasmic loops and termini of MFSD4A may interact with signaling molecules that modulate transporter activity in response to nutrient status or hormonal cues. Expression profiling reveals that MFSD4A is enriched in liver, pancreas, and brain tissues, consistent with its proposed role in metabolic homeostasis and neuronal energy regulation.
Functionally, MFSD4A may participate in glucose or amino acid transport pathways and contribute to the regulation of insulin secretion and energy metabolism. Emerging transcriptomic data indicate that MFSD4A expression is responsive to fasting, insulin signaling, and circadian rhythm regulators. In neurons, it may be involved in neurotransmitter or metabolite shuttling across synaptic membranes. Given its homology with nutrient transporters, MFSD4A is under study as a potential metabolic sensor that links energy availability to intracellular signaling pathways such as mTOR and AMPK.
From a structural and evolutionary perspective, MFSD4A belongs to the major facilitator superfamily, which includes transporters for sugars, ions, and small molecules. Members of this family typically share conserved sequence motifs such as the MFS signature (GXXXDRXGRR) involved in proton coupling and conformational switching. These features support the hypothesis that MFSD4A mediates solute exchange across cell membranes. Its expression in metabolically active tissues suggests integration with pathways governing glucose utilization and lipid metabolism.
Dysregulation of MFSD4A has been observed in metabolic disorders and certain cancers. Genome analyses have identified associations between MFSD4A variants and altered plasma metabolite levels, insulin resistance, and hepatocellular carcinoma progression. In the nervous system, perturbations in MFSD4A expression may influence neuronal excitability and cognitive function through metabolic imbalance. The protein's role in membrane transport and nutrient sensing positions it as a potential target for metabolic and neurological research.
Immunohistochemical staining using MFSD4A antibody reveals membrane localization in hepatocytes, pancreatic islets, and neurons. MFSD4A antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
