| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human C4orf49/MGARP recombinant protein (Position: M1-E155) was used as the immunogen for the MGARP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MGARP Antibody / Mitochondria-localized glutamic acid-rich protein is a anti-MGARP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MGARP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
MGARP is encoded by the MGARP gene located on human chromosome 4q28.1. The protein is approximately 25 kilodaltons in size and characterized by an N-terminal mitochondrial targeting sequence and two C-terminal transmembrane domains that anchor it to the outer mitochondrial membrane. It interacts with proteins involved in mitochondrial motility and positioning, including Miro, TRAK1, and kinesin motor complexes. Through these interactions, MGARP mediates mitochondrial trafficking along microtubules and maintains energy homeostasis in neurons and photoreceptors.
The MGARP antibody is typically used to identify mitochondrial localization via immunofluorescence microscopy, where it produces a distinct punctate pattern consistent with the mitochondrial network. Western blot analysis detects a band near 38 kilodaltons. MGARP expression is enriched in neural tissues, endocrine organs, and energy-demanding cell types. Experimental evidence shows that it modulates mitochondrial fission and fusion dynamics, contributing to the distribution of mitochondria within axons and synaptic terminals. Loss or dysregulation of MGARP disrupts mitochondrial motility and impairs neuronal differentiation, emphasizing its importance in developmental neurobiology.
Beyond neuronal systems, MGARP has been implicated in steroid hormone biosynthesis and cellular stress adaptation. In steroidogenic cells, MGARP expression is upregulated by cAMP signaling and may influence mitochondrial cholesterol import. Its expression is responsive to hypoxia, oxidative stress, and glucose deprivation, indicating a role in cellular adaptation to metabolic stress.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
