| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | Recombinant human protein (amino acids A71-I572) was used as the immunogen for the MIB2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MIB2 Antibody / Mindbomb 2 is a anti-MIB2 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: MIB2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Antigen affinity purified
- Applications (as listed): WB, FACS, IP
Biological background
Beyond canonical Notch signaling, MIB2 has been implicated in cytoskeletal organization and broader ubiquitin-mediated quality control. Altered MIB2 expression or activity can influence cell fate decisions, neural and immune system function, and tumor biology, where disrupted ligand trafficking and signaling balance contribute to disease phenotypes. Its functional overlap and divergence relative to the paralog MIB1 highlight MIB2 as a distinct regulator with tissue- and context-specific roles.
The MIB2 antibody enables specific detection of endogenous MIB2 in cells and tissues for applications including western blot, immunofluorescence, and immunohistochemistry. Researchers use the MIB2 antibody to quantify expression, assess subcellular localization at membranes and endosomes, and monitor pathway modulation under genetic or pharmacologic perturbations. With high specificity and consistent performance, the MIB2 antibody supports studies of ubiquitin signaling, Notch pathway regulation, and disease mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
