| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MICALL1 recombinant protein (Position: R192-K747) was used as the immunogen for the MICALL1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MICALL1 Antibody / MICAL-like 1 is a anti-MICALL1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: MICALL1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
MICALL1 is encoded by the MICALL1 gene located on human chromosome 22q13.1. The protein is approximately 94 kilodaltons and includes a calponin homology (CH) domain, a LIM domain, and multiple coiled-coil regions that mediate binding to Rab GTPases and the EHD family of endocytic regulators. MICALL1 localizes to tubular recycling endosomes, where it coordinates membrane curvature and receptor sorting.
The MICALL1 antibody detects a 94 kilodalton protein by western blot and shows punctate cytoplasmic staining in immunofluorescence. MICALL1 interacts with Rab8, Rab11, and EHD1, forming a complex that facilitates recycling of receptors such as transferrin and integrins to the plasma membrane. It also binds to actin regulatory proteins, linking vesicle trafficking to cytoskeletal rearrangements and cell morphology maintenance.
Disruption of MICALL1 expression impairs membrane recycling and causes accumulation of endosomal tubules. Mutations in MICALL1 are associated with autosomal recessive neurodevelopmental disorders characterized by ataxia and delayed myelination. In epithelial cells, MICALL1 participates in maintaining tight junction integrity and apical-basal polarity, processes essential for tissue organization.
Because of its central role in coordinating membrane transport, MICALL1 contributes to diverse biological pathways, from nutrient uptake to synaptic vesicle recycling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
