| Field | Specification |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human MID2 was used as the immunogen for the MID2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MID2 Antibody / Midline 2 is a anti-MID2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MID2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS
Biological background
In human cells, MID2 localizes along microtubules, at the centrosome, and in perinuclear regions. It associates with its close paralog MID1, with which it shares structural and functional similarities. Together, MID1 and MID2 regulate the stability of PP2A catalytic subunits and participate in tubulin network maintenance. MID2 expression is particularly high in brain, testis, and embryonic tissues, reflecting roles in neuronal migration and organ development. Mutations in MID2 have been associated with X-linked intellectual disability and developmental disorders affecting craniofacial and skeletal structures.
The MID2 antibody is widely used in cell biology and developmental research to study microtubule organization, ubiquitination, and cell morphology. Western blot analysis detects a ~78 kilodalton band corresponding to full-length MID2, while immunofluorescence reveals filamentous staining patterns overlapping with microtubules. MID2 ubiquitinates several substrates involved in cytoskeletal remodeling and signaling, including MID1-associated proteins and small GTPase regulators. Through these activities, it influences cell polarity, division, and migration. In neurons, MID2 contributes to axon guidance and growth cone dynamics.
Dysregulation of MID2 expression or mutation of its functional domains can lead to developmental abnormalities and neurological defects. Overexpression has been observed in certain cancers, where it may enhance cell motility and invasiveness through altered cytoskeletal signaling. The MID2 antibody provides a robust tool for investigating these pathways and identifying mechanisms of microtubule regulation in both normal and diseased states.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
