| Field | Specification |
|---|---|
| Expression System | |
| Form | Liquid |
| Product Type | |
| Reporter | |
| Storage | |
| Target |
Scientific Background
Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a betacoronavirus first identified in Saudi Arabia in 2012. With a case fatality rate of approximately 35%, MERS-CoV remains a significant public health concern and a pandemic threat. MERS-CoV uses dipeptidyl peptidase-4 (DPP4/CD26) as its receptor, and the spike protein mediates viral entry. Safe, quantitative tools for studying MERS-CoV entry and evaluating anti-MERS countermeasures are essential for research and drug development.
Product Description
Coronavirus
This Ha-MERS-CoV VLP is a hybrid alpha-pseudovirus assembled from all four MERS-CoV structural proteins (S, M, N, E) with an alphavirus-derived reporter RNA payload. Single-cycle, BSL-2 safe. Available with Firefly luciferase, GFP, or RFP reporter. 60X concentrated format.
Product Specifications
| Catalog Number | 0569-PP-002 |
|---|---|
| Full Name | MERS-CoV Virus-Like Particles (Ha-MERS-CoV), Wild Type |
| Structural Proteins | S (spike), M (membrane), N (nucleocapsid), E (envelope) |
| Reporter Payload | Alphavirus-derived self-replicating RNA encoding Firefly Luciferase, GFP, or RFP |
| Particle Type | Single-cycle alpha-pseudovirus (BSL-2 safe) |
| Target Cells | DPP4/CD26-expressing cells (e.g., Vero cells, HEK293T-DPP4) |
| Form | Liquid |
| Size | 60X Concentrated: 5 × 100 µL |
| Storage | -80°C. Avoid repeated freeze-thaw cycles. |
Applications
- Screening and quantification of anti-MERS-CoV neutralizing antibodies
- Anti-MERS-CoV drug and entry inhibitor screening
- Identification of host co-factors for MERS-CoV entry
Safety & Handling
Ha-MERS-CoV uses the same hybrid alpha-pseudovirus platform as Ha-CoV-2, but presents the MERS-CoV spike protein and is assembled with all four MERS-CoV structural proteins (S, M, N, E). It targets DPP4/CD26 rather than ACE2 as the entry receptor.
BSL-2 containment is required. Ha-MERS-CoV VLPs are single-cycle particles that cannot produce infectious MERS-CoV. Standard BSL-2 precautions apply.
Firefly luciferase is recommended for HTS and dose-response neutralization assays. GFP or RFP are suitable for imaging-based or flow cytometry-based entry studies.
Need a different reporter, pack size, or bulk volume? BioHippo works directly with IBT Bioservices to support custom and large-volume requests. Contact us for a quote or to discuss your project requirements.
Looking for matching antigen proteins, antibodies, or other coronavirus/filovirus research reagents? Contact BioHippo support for recommendations.
Research budgets are tight — we get it. That's why we've put together a fresh round of exclusive promotions designed to help you stock up on the reagents, kits, and consumables your lab depends on, without stretching your budget.
🔬 What's on offer right now:
10% Off Pre-Designed siRNA Sets
20% Off Transmembrane Proteins
50% Off Lab Consumables + Free Shipping
$99 Pipette Filler Promotion Package
BlasTaq 2X qPCR MasterMix - 50% OFF Limited Time Offer
DENARASE® Endonuclease — 10% Off One Order
10% OFF CELL LINES-Limited-Time Offer
