| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Puromycin |
| Shipping | |
| Species |
Background
miR-199a-5p is the mature microRNA derived from the MIR199A1 stem-loop and is conserved between human and mouse. It is generated through the canonical microRNA pathway, in which a primary transcript is processed by Drosha/DGCR8 and Dicer and the mature strand is loaded into the RNA-induced silencing complex to repress target mRNAs. miR-199a-5p regulates genes involved in cell proliferation, migration, extracellular matrix remodeling, and stress responses. Through these targets it has been studied in tissue fibrosis, cardiovascular biology, and as a context-dependent regulator of tumor cell behavior, making it a useful tool for investigating microRNA-mediated gene control.
Product Description & Applications
This miRNA lentivirus delivers the complete human miR-199a stem-loop, which is processed in transduced cells to yield the mature miR-199a-5p sequence common to human and mouse. The stem-loop is driven by a U6 promoter, while a PGK promoter drives a GFP reporter and a puromycin selection marker separated by a self-cleaving peptide, allowing transduced cells to be identified by fluorescence and enriched by antibiotic selection or FACS.
The particles are ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation, supporting in vitro and in vivo use and transduction of difficult-to-transfect cells such as primary and thawed cultures. Polyclonal stable cell lines can be established within about 10 days for long-term studies of miR-199a-5p function and microRNA biology while preserving parental cell properties.
About This Product
This lentivirus expresses the full pre-miRNA stem-loop sequence of MIR199A1 under a Pol II promoter (CMV or EF1α), enabling endogenous Drosha/DGCR8 and Dicer processing to produce authentic mature 5P (and 5p) strands loaded into RISC. This produces physiologically relevant miRNA activity that faithfully replicates the endogenous biogenesis pathway, unlike synthetic miRNA mimics that bypass nuclear processing and can exhibit RISC overloading artifacts.
Co-expression of a fluorescent reporter (GFP) and selection marker (Puromycin) allows stable transductant identification by fluorescence and antibiotic enrichment. Stable miRNA overexpression enables long-term target repression studies, in vivo tumor models, and exosomal miRNA loading experiments that are not achievable with transient miRNA mimics.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
