| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A semi-purified mitochondrial preparation was used as the immunogen for this Mitochondrial marker antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target |
Overview
Mitochondrial Marker Antibody is a research-use primary antibody intended for detection of MITOCHONDRIAL in experimental workflows. It is supplied in Purified format. Key antibody attributes include Mouse, Monoclonal (mouse origin), clone 113-1, isotype Mouse IgG1, kappa. Applications listed for this product include IF, WB, IHC-P. Reported/annotated localization context: Mitochondria in cytoplasm. Species reactivity (as provided): Human.
Key elements and design rationale
- Target: MITOCHONDRIAL (Mitochondrial Marker) — selectivity and interpretation should be considered in the context of isoforms, post-translational modifications, and related family members when applicable.
- Format: Purified — format can influence background, multiplexing compatibility, and downstream detection strategies.
- Antibody identity: Mouse, Monoclonal (mouse origin), clone 113-1, isotype Mouse IgG1, kappa — these attributes help align secondary reagents and controls (e.g., isotype-matched controls) with your assay design.
- Localization: Mitochondria in cytoplasm — expected subcellular distribution can guide band/structure interpretation and help flag off-target signal.
- Product notes (from provided description): Clone 113-1 antibody recognizes a 60kDa antigen that is a mitochondrial marker in human cells. Immunostaining produces a spaghetti-like pattern in normal and malignant cells. This mitochondrial marker antibody is an excellent marker for human cells in xenographic model research. Abundant granular eosinophilic cytoplasm is a common feature of renal oncocytoma, chromophobe renal cell carcinoma, eosinophilic variant of papillary renal cell carcinoma, and the granular variant of clear cell renal cell carcinoma (RCC). The chief reason for their distinction from one another is the difference in their biologic behavior. However, precise characterization may be difficult in some cases because of overlapping morphologic features. Immunostaining pattern with this mitochondrial marker antibody has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Salivary gland tumors usually show great variability both in their morphopathology as well as their clinical behavior. One study highlights the usefulness of this mitochondrial marker antibody to facilitate the classification of salivary tumors, an aspect that may sometimes have not only diagnostic implications, but also prognostic.
Where multiple assay formats are possible, align the antibody format, host/isotype, and listed applications with your detection system and controls to support clear interpretation of signal.
Biological background
In this catalog, MITOCHONDRIAL is positioned within Renal & Urology, Tumor, Renal disease research contexts. Localization annotations (e.g., Mitochondria in cytoplasm) can help contextualize expected signal patterns in imaging and fractionation-based readouts. For authoritative gene/protein nomenclature, domains/isoforms, and curated functional annotations, consult resources such as UniProt, NCBI Gene, and Ensembl.
Research relevance and current trends
- Higher-plex and spatially resolved readouts (e.g., multiplex IF/IHC, spatial omics) are increasing demand for well-characterized primary antibodies with clearly stated host/isotype and labeling strategies.
- Genetic perturbation controls (knockout/knockdown) and orthogonal measurements (e.g., RNA vs protein) are commonly used to strengthen target attribution when interpreting antibody-derived signals.
- Reproducibility initiatives emphasize transparent reporting of antibody identity (clone, host, isotype) and experimental context to improve cross-study comparability.
Common research applications
- IF: interpret changes in signal in the context of sample composition, epitope accessibility, and potential isoform/PTM differences across conditions.
- WB: interpret changes in signal in the context of sample composition, epitope accessibility, and potential isoform/PTM differences across conditions.
- IHC-P: interpret changes in signal in the context of sample composition, epitope accessibility, and potential isoform/PTM differences across conditions.
- Typical workflow themes: Western blot validation, IHC on FFPE tissue, IF/ICC localization, Specificity controls.
- Workflow notes: Validate MITOCHONDRIALMA by Western blot in cell/tissue lysates (include controls), Detect MITOCHONDRIALMA by IHC in FFPE tissue sections (optimize antigen retrieval + dilution), Detect MITOCHONDRIALMA localization by…
When comparing conditions, consistent sample processing and appropriate negative/positive controls support interpretation of qualitative localization differences and quantitative abundance changes.
Notes for experimental interpretation
- Isoforms and post-translational modifications may shift apparent molecular weight or epitope accessibility, especially across cell states or treatments.
- Species and tissue context can affect sequence conservation, expression level, and background binding; predicted reactivity should be verified in your sample.
- Control concepts include isotype-matched controls, secondary-only controls (for indirect detection), and genetic/orthogonal controls (e.g., KO/KD, independent antibodies, or RNA measurements) when feasible.
Monoclonal and polyclonal antibodies can differ in epitope recognition breadth and lot-to-lot characteristics; consider clonality and clone information (when provided) alongside your assay requirements. Conjugated formats may simplify detection but can change background and multiplexing behavior compared with unconjugated primaries.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
