| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | MLH1; COCA2; DNA mismatch repair protein Mlh1; MutL protein homolog 1 |
| Clonality | |
| Concentration | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli. |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
Primary AntibodyThis antibody targets MLH1 and detects endogenous levels of MLH1 protein.
Validated Applications
| Application | Dilution Guidance |
|---|---|
| Western Blot (WB) | Refer to datasheet for recommended working dilution |
| Immunohistochemistry (IHC) | Refer to datasheet for recommended working dilution |
| Immunofluorescence (IF) | Refer to datasheet for recommended working dilution |
| Immunocytochemistry (ICC) | Refer to datasheet for recommended working dilution |
| ELISA | Refer to datasheet for recommended working dilution |
Antibody Specifications
| Target | MLH1 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Reactivity | Human, Monkey |
| Purification | Affinity Purification |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol. |
| Storage | -20°C for 1 year |
| Gene ID | Human: 4292 |
| UniProt | P40692 (Human: P40692; Mouse: Q9JK91) |
Scientific Background
This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.
Alternative Names
MLH1; COCA2; DNA mismatch repair protein Mlh1; MutL protein homolog 1
Safety & Handling
| Target | MLH1 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Reactivity | Human, Monkey |
| Purification | Affinity Purification |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol. |
| Storage | -20°C for 1 year |
| Gene ID | Human: 4292 |
| UniProt | P40692 (Human: P40692; Mouse: Q9JK91) |
Optimized working dilutions depend on sample type and detection system. Titration is recommended.
| Application | Dilution Note |
|---|---|
| Western Blot (WB) | Refer to datasheet for recommended working dilution |
| Immunohistochemistry (IHC) | Refer to datasheet for recommended working dilution |
| Immunofluorescence (IF) | Refer to datasheet for recommended working dilution |
| Immunocytochemistry (ICC) | Refer to datasheet for recommended working dilution |
| ELISA | Refer to datasheet for recommended working dilution |
This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.
Alternative Names
MLH1; COCA2; DNA mismatch repair protein Mlh1; MutL protein homolog 1
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
