| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A recombinant human partial protein was used as the immunogen for the MLL3 antibody. |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MLL3 Antibody / KMT2C is an antibody targeting KMT2C, raised in Rabbit for protein detection and localization studies where these specifications are required.
Key elements and design rationale
- Target: KMT2C.
- Antibody identity: Monoclonal (mouse origin); Clone 1686CT202.60.69; Mouse IgG1, kappa.
- Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
- Format: Purified.
- Species reactivity: Human.
- Listed applications: WB (refer to on-page specifications for application-specific guidance).
Biological background
KMT2C (Lysine N-methyltransferase 2C), also called MML3 (Myeloid/lymphoid or mixed-lineage leukemia protein 3) is a histone methyltransferase, which means it adds methyl groups to histone proteins. This modification plays a critical role in controlling how genes are turned on and off. By regulating gene expression, KMT2C/MML3 can influence various cellular processes, including cell growth, differentiation, and death. Research has also shown that mutations in the KMT2C gene are associated with the development of certain types of cancer. For example, mutations in KMT2C have been found in breast cancer, colorectal cancer, and other types of tumors. In addition to its role in cancer, KMT2C/MML3 is also involved in DNA repair. This protein plays a crucial role in repairing damaged DNA, which is essential for maintaining genomic stability and preventing the development of genetic mutations.
Research relevance and current trends
- Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
- Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
- Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.
Common research applications
- Western blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.
Notes for experimental interpretation
- Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
- Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
- Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
- Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
