| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Melanophilin/MLPH recombinant protein (Position: E61-L523) was used as the immunogen for the MLPH antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MLPH Antibody / Melanophilin is a anti-MLPH Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: MLPH
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, ELISA
Biological background
Structurally, Melanophilin is a 590-amino-acid cytoplasmic protein of approximately 67 kilodaltons containing an N-terminal Rab27A-binding domain, a central actin- and myosin Va-interacting region, and a C-terminal coiled-coil domain that stabilizes the tripartite complex. This structural organization enables MLPH to bridge the small GTPase Rab27A and the motor protein myosin Va, coordinating microtubule and actin-based transport systems. In pigment cells, MLPH localizes to melanosome membranes and cortical actin filaments where it regulates vesicle tethering and release.
The MLPH antibody is widely used in cell biology, dermatology, and neurobiology research to study vesicle trafficking, motor protein coordination, and pigment cell organization. Western blot analysis detects a 67 kilodalton band corresponding to Melanophilin, while immunofluorescence reveals peripheral punctate staining in melanocytes, consistent with melanosomal distribution. This antibody is ideal for examining organelle transport mechanisms and intracellular vesicle dynamics in epithelial and neuronal systems.
Mutations in MLPH cause Griscelli syndrome type 3, a rare pigmentation disorder characterized by hypopigmentation and abnormal melanosome aggregation due to disrupted melanosome transport. Functional studies show that loss of MLPH leads to perinuclear melanosome clustering, highlighting its importance in vesicle tethering to the actin cytoskeleton. Beyond pigmentation, MLPH has been implicated in secretory vesicle dynamics in non-pigment cell types, suggesting a broader role in vesicle trafficking and cytoskeletal coupling. The MLPH antibody provides a reliable tool for characterizing this transport system and investigating the molecular basis of vesicle transport defects.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
