| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MMP20 recombinant protein (Position: Y124-K405) was used as the immunogen for the MMP20 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MMP20 Antibody / Matrix metalloproteinase 20 is a anti-MMP20 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: MMP20
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, MMP20 antibody identifies a 483-amino-acid secreted zinc-dependent protease synthesized as a zymogen that is activated by propeptide cleavage. MMP20 is specifically expressed in ameloblasts and odontoblasts, where it cleaves amelogenin, ameloblastin, and enamelin to enable proper enamel mineralization and organization.
The MMP20 gene is located on chromosome 11q22.2 and exhibits tissue-specific expression limited primarily to developing teeth. Its enzymatic activity contributes to normal enamel formation and maturation during odontogenesis.
Pathologically, mutations in MMP20 cause autosomal recessive amelogenesis imperfecta, a disorder characterized by defective enamel formation and tooth fragility. Altered MMP20 expression has also been implicated in oral cancer and tissue invasion. Research using MMP20 antibody supports studies in dental biology, extracellular matrix remodeling, and developmental biochemistry.
MMP20 antibody is validated for western blotting, immunohistochemistry, and ELISA to detect matrix-degrading enzymes.
Structurally, Matrix metalloproteinase-20 features a catalytic zinc-binding domain and a hemopexin-like domain responsible for substrate recognition. This antibody enables investigation of MMP20's function in enamel formation and extracellular matrix turnover.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
