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| Sample Type(s) | Plasma, Serum |
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Overview
Monkeypox Virus (MPXV) A35R Antigen ELISA Kit is an ELISA-based immunoassay designed for quantitative measurement of A35R (Monkeypox Virus (MPXV)) in research samples. It is commonly used to generate traceable concentration data for biomarker discovery, pathway studies, and comparative analyses across experimental conditions.
Key elements and design rationale
- Assay format: Quantitative Colorimetric ELISA. The format defines how signal scales with analyte abundance and how results are interpreted across a standard curve.
- Working range and sensitivity: dynamic range 375 pg/mL - 24,000 pg/mL; analytical sensitivity 158.4 pg/mL. Use these values to plan dilutions and keep readouts within the linear portion of the calibration curve.
- Sample compatibility: Intended for Plasma, Serum matrices. As with most immunoassays, matrix composition can influence apparent signal and should be evaluated with dilution linearity and spike-recovery concepts.
- Recovery reference: Typical recovery is reported as 70-100%. Recovery helps assess whether the sample matrix interferes with detection of spiked analyte.
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Monkeypox Virus A35R Antigen has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Monkeypox Virus A35R Antigen present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled Monkeypox Virus A35R antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Monkeypox Virus A35R Antigen bound in the initial step. The color development is stopped and the intensity of the color is measured.
Biological background
A35R is a commonly studied research analyte. Its biological roles and regulation can be context-dependent (cell type, tissue state, and experimental perturbation). For authoritative gene/protein function summaries and nomenclature, researchers typically consult curated databases.
Research relevance and current trends
- Biomarker translation in RUO settings: Increasing use of quantitative immunoassays to stratify experimental cohorts, track longitudinal changes, and benchmark model systems.
- Matrix-aware assay design: Greater emphasis on dilution linearity, spike-recovery, and control concepts to reduce matrix-driven artifacts in serum/plasma and complex lysates.
- Integration with multi-omics: ELISA measurements are often used alongside transcriptomics and proteomics to connect abundance changes with pathway activity and phenotype.
Common research applications
- Comparative quantification: Measure relative changes in analyte levels across treatments, time points, or genotypes to support mechanistic hypotheses.
- Assay development and standardization: Generate reproducible concentration inputs for method qualification, inter-operator comparisons, or bridging studies across platforms.
- Model and sample characterization: Profile baseline and stimulated levels to help interpret immune, endocrine, neurodegenerative, or metabolic phenotypes (as relevant to the target).
Interpretation typically focuses on direction and magnitude of change in the context of controls and sample handling metadata, rather than single-point absolute values.
Notes for experimental interpretation
- Matrix effects: Hemolysis, lipemia, and high protein content can alter background and apparent concentration. Consider consistent collection/processing and evaluate dilution behavior.
- Isoforms and modified forms: Some targets exist as isoforms, fragments, or post-translationally modified species. Ensure the measured form aligns with the biological question and the kit’s intended analyte definition.
- Control concepts: Use negative/blank controls, replicate wells, and—when feasible—orthogonal confirmation (e.g., WB or MS) to strengthen conclusions.
What does this ELISA kit measure?
This sandwich ELISA kit is designed to quantitatively measure A35R in biological samples. It uses a validated antibody pair with high specificity for the target antigen, making it suitable for research in Infectious Disease applications.
What sample types are compatible with this kit?
This kit has been validated for use with Plasma and Serum. Samples should be diluted as recommended in the protocol to fall within the standard curve range. Cross-reactivity with other matrices has not been systematically evaluated and should be tested by the user.
What is the detection range and sensitivity of this kit?
The standard curve covers 375 pg/mL - 24,000 pg/mL. The minimum detectable concentration (sensitivity/LLOD) is 158.4 pg/mL. Samples with expected concentrations outside this range should be diluted or concentrated accordingly.
How should I prepare my samples before running the assay?
Samples should be collected, processed, and stored according to established best practices for the matrix type. Avoid repeated freeze-thaw cycles. Centrifuge samples containing particulates at 2,000?3,000 ? g for 10 minutes. Dilute samples in the provided assay diluent and run in duplicate. Refer to the kit insert for recommended dilution factors.
Can this kit be used for clinical diagnostic purposes?
No. This kit is intended for research use only (RUO) and is not approved or validated for clinical diagnosis, patient management, or therapeutic monitoring. Results obtained with this kit should not be used to make clinical decisions.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
