| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Polyclonal IgY (Promega) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage |
Product Overview
Secondary Antibody
This mouse monoclonal antibody (Clone 08C, IgG1) is an anti-Chicken IgG (IgY) secondary antibody reactive with both heavy and light chains of Chicken IgG. Raised using polyclonal chicken IgY (Promega) as immunogen. Supplied lyophilized for extended shelf life and shipping stability. Detects chicken-derived primary antibodies in ELISA and WB.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| ELISA | Optimize experimentally |
| Western Blot | Optimize experimentally |
Antibody Specifications
| Host Species | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone | 08C |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Reactivity | Chicken IgG (IgY) — heavy and light chain |
| Formulation | Lyophilized in 10 mM ammonium buffer |
| Storage | 2–8°C (lyophilized); −20°C after reconstitution (aliquoted) |
| Mfr Catalogue No. | STJ16100953 |
Immunogen & Specificity
Immunogen: Polyclonal chicken IgY (Promega) — heavy and light chain immunogen.
Specificity: Reacts with Chicken IgG (IgY) heavy and light chains. Does not cross-react with mammalian IgG.
Reactivity
Specific for Chicken IgG (IgY). Use as secondary antibody when the primary is Chicken-derived.
Safety & Handling
Research Use Only (RUO). Not for diagnostic or therapeutic applications. Lyophilized in 10 mM ammonium buffer — reconstitute per instructions. RUO only.
Related Products
Browse additional antibodies and detection reagents in the BioHippo catalog.
This secondary detects Chicken-derived (IgY) primary antibodies in immunoassays. When your primary antibody is from a chicken source, this Mouse anti-Chicken IgG provides the detection bridge for colorimetric, chemiluminescent, or fluorescent readout.
No. This antibody is specific for Chicken IgG (IgY) and does not cross-react with mammalian IgG (Mouse, Rabbit, Human, Goat, etc.). This specificity makes it ideal for multiplex experiments with both Chicken-derived and mammalian primary antibodies.
Reconstitute in sterile water or PBS to the volume indicated on the CoA to achieve the target concentration. Mix gently — do not vortex. Allow 5–10 minutes at room temperature before use. Prepare single-use aliquots and store at −20°C.
Since this antibody is unconjugated Mouse IgG1, pair with an anti-Mouse IgG secondary conjugated to HRP (for ELISA/WB) or fluorophore (for IF). This creates: Chicken primary → Mouse anti-Chicken secondary → Anti-Mouse tertiary detection.
Validated for ELISA and Western Blot (Immunoblot). Optimal dilutions must be determined empirically for each application, sample type, and detection system.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
