| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Horseradish peroxidase (Grade I) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage |
Product Overview
Secondary Antibody
This mouse monoclonal antibody (Clone HP-3, IgG1) targets horseradish peroxidase (HRP) and is itself conjugated to HRP for direct enzymatic detection. It enables detection of HRP-tagged proteins without a secondary antibody, simplifying HRP-based immunodetection workflows in WB, ICC/IF, and ELISA formats.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Western Blot | Optimize experimentally |
| Immunofluorescence | Optimize experimentally |
| ELISA | Optimize experimentally |
Antibody Specifications
| Host Species | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone | HP-3 |
| Isotype | IgG1 |
| Conjugate | HRP (Horseradish Peroxidase) |
| Reactivity | Horseradish Peroxidase (HRP) / HRP-tagged molecules |
| Concentration | 1 mg/mL |
| Formulation | PBS with 15 mM sodium azide |
| Storage | 2–8°C, up to 1 year — DO NOT FREEZE |
| Mfr Catalogue No. | STJ16100611 |
Immunogen & Specificity
Immunogen: Horseradish peroxidase (Grade I) — native HRP enzyme used as immunogen.
Specificity: Reacts with horseradish peroxidase (HRP) and HRP-labeled proteins/conjugates.
This antibody is conjugated to HRP (Horseradish Peroxidase) — direct detection without secondary antibody.
Reactivity
HRP-specific; tag-based. Not species-dependent.
Safety & Handling
Research Use Only (RUO). Not for diagnostic or therapeutic applications. Buffer contains 15 mM sodium azide — toxic; avoid ingestion. HRP substrates (TMB, DAB, ECL) require handling per institutional guidelines.
Related Products
Browse additional antibodies and detection reagents in the BioHippo catalog.
No. This antibody is directly HRP-conjugated — it enables direct enzymatic detection without a secondary antibody. After blocking, add the antibody, wash, and then add your HRP substrate (TMB for ELISA, ECL for WB).
Validated for Western Blot (WB), Immunofluorescence/ICC, and ELISA. Working dilutions must be optimized empirically for each application and sample type.
For WB: use an ECL (enhanced chemiluminescence) reagent compatible with HRP. For ELISA: use TMB with a sulfuric acid stop solution. For IHC/IF: use DAB (3,3′-diaminobenzidine) for chromogenic detection or tyramide-based fluorescent signal amplification.
Store at 2–8°C upon receipt. Stable for up to 1 year. Do not freeze — HRP-conjugated antibodies lose activity upon freeze-thaw cycling. The HRP enzyme is inactivated by freezing.
Yes. This anti-HRP antibody detects any HRP-conjugated molecule, including HRP-conjugated secondary antibodies and streptavidin-HRP conjugates, making it useful as a positive control or in ELISA-based HRP detection formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
