Mouse Anti-Human IgG Fc Fragment antibody {Unconjugated} (STJ98147)

What it means: Human IgG Fc Fragment — target for this product.

Why it matters:

• Fc-fragment specificity means this antibody detects all Human IgG subclasses (IgG1–IgG4) via their conserved constant region, without cross-reacting with the antigen-binding Fab region.
• Useful for quantifying total Human IgG, detecting anti-drug antibodies (ADA), or as a secondary antibody for human primary antibody detection.

How to interpret here: This antibody detects ALL Human IgG molecules via their Fc region — useful when you need pan-Human IgG detection regardless of antigen-binding specificity.

What it means: WB 1:500–2,000 / IF 1:200–1,000 / ELISA 1:10,000 — validated dilutions for this product.

Why it matters:

• Pre-validated dilutions across three applications (WB, IF, ELISA) provide confirmed starting points, reducing assay development time.
• ELISA validation at 1:10,000 indicates high sensitivity, suitable for detecting trace amounts of Human IgG.

How to interpret here: Start at WB 1:1,000, IF 1:500, and ELISA 1:10,000. Fine-tune based on your specific sample, detection system, and desired signal-to-noise ratio.

What it means: Affinity Purified — purification for this product.

Why it matters:

• Affinity purification removes non-specific immunoglobulins, providing higher purity and lower background compared to crude antisera.
• Defined purity enables more accurate working dilution calculations and supports quantitative ELISA applications.

How to interpret here: The affinity-purified format supports reliable quantitative applications such as ELISA and quantitative WB at defined, reproducible concentrations.

What it means: −20°C — storage temperature for this product.

Why it matters:

• Long-term storage at −20°C maintains antibody activity for up to 1 year from receipt.
• The 50% glycerol formulation prevents freezing at −20°C, allowing direct pipetting without thawing — unlike standard frozen antibodies.

How to interpret here: Store at −20°C immediately upon receipt. The 50% glycerol prevents freeze-solidification — pipette directly from −20°C storage. Avoid transferring to 4°C repeatedly.

What it means: Monoclonal (Clone 4A10) — clonality for this product.

Why it matters:

• Clone 4A10 provides highly reproducible, defined Fc-specific recognition with consistent performance across lots — critical for quantitative Human IgG ELISA and ADA assays.
• Monoclonal format enables standardized assay development with minimal inter-assay variability.

How to interpret here: Validate dilutions (WB 1:500–2,000; IF 1:200–1,000; ELISA 1:10,000) with the first lot. Monoclonal consistency ensures these dilutions remain valid across subsequent lots.