| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | ME20M, Melanocyte protein PMEL, Silver locus protein homolog, Secreted melanoma-associated ME20 antigen, D12S53E, 95 kDa melanocyte-specific secreted glycoprotein, SILV, P100, Premelanosome protein, PMEL17, ME20S, PMEL, ME20-S, ME20-M, P26, P1, Melanocytes lineage-specific antigen GP100, Melanocyte protein Pmel 17, Melanoma-associated ME20 antigen |
| Clonality | |
| Concentration | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target |
Overview
Mouse Anti-Human PMEL17/ME20M Antibody (17A9) targets PMEL17 (95 kDa melanocyte-specific secreted glycoprotein). Frequently used across ELISA, FCM, IHC workflows, depending on experimental design and sample type. This antibody is suited for workflows commonly used in Molecular & Cellular Biology research.
Also known as: 95 kDa melanocyte-specific secreted glycoprotein, Melanocytes lineage-specific antigen GP100, Secreted melanoma-associated ME20 antigen, Silver locus protein homolog, Melanocyte protein Pmel 17, Melanocyte protein PMEL, Melanoma-associated ME20 antigen, Premelanosome protein, ME20-S, ME20-M.
Target biology
PMEL17 (95 kDa melanocyte-specific secreted glycoprotein) is a pathogen-derived antigen commonly used to detect infection, antigen expression, or immune responses in research assays. Envelope/glycoprotein antigens are often surface-exposed and may be assessed for expression, trafficking, and antibody recognition in cell-based systems. Assay performance can depend on native conformation, glycosylation, and sample preparation conditions. Common alternative names and symbols used in the literature include: Melanocytes lineage-specific antigen GP100, Secreted melanoma-associated ME20 antigen, Silver locus protein homolog, Melanocyte protein Pmel 17, Melanocyte protein PMEL, Melanoma-associated ME20 antigen, Premelanosome protein, ME20-S, ME20-M, D12S53E. Based on the annotated name/class, the target is most often associated with secreted / extracellular localization, though this can vary by model system.
How this antibody helps
As a monoclonal reagent, this antibody is designed for clone-to-clone consistency and epitope-focused recognition. For best performance, interpret signal in the context of appropriate biological controls and orthogonal readouts when available.
Recommended experimental notes
- Titrate the antibody to optimize signal-to-noise for your specific sample type and detection system.
- Use appropriate positive and negative controls (e.g., target-high vs target-low samples, KO/KD where available).
- For IHC, optimize fixation and antigen retrieval (buffer/pH/heat) and include an isotype/secondary-only control to assess background.
- For ELISA, optimize capture/detection pairing and run a standard curve in matrix-matched diluent when possible.
For research use only. Not for diagnostic procedures.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
