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Product Overview
Secondary Antibody
This mouse monoclonal antibody (Clone Bio-8, IgG3) against tag proteins is conjugated to biotin, enabling high-sensitivity amplified detection via streptavidin-enzyme or streptavidin-fluorophore systems. The biotin–streptavidin system (Kd ~10⁻¹⁵ M) provides exceptional signal amplification for IHC, ELISA, and flow cytometry workflows.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Immunohistochemistry | Optimize experimentally |
| ELISA | Optimize experimentally |
| Flow Cytometry | Optimize experimentally |
Antibody Specifications
| Host Species | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone | Bio-8 |
| Isotype | IgG3 |
| Conjugate | Biotin |
| Reactivity | Tag proteins |
| Concentration | 100 µg/mL |
| Formulation | Tissue culture supernatant with sodium azide |
| Storage | 2–8°C, up to 1 year |
| Mfr Catalogue No. | STJ16100612 |
Immunogen & Specificity
Immunogen: Tag protein antigen (proprietary).
Specificity: Reacts with tag proteins; Clone Bio-8.
This antibody is conjugated to Biotin — direct detection without secondary antibody.
Reactivity
Tag-specific; species-independent.
Safety & Handling
Research Use Only (RUO). Not for diagnostic or therapeutic applications. Formulation contains sodium azide — avoid ingestion. RUO only.
Related Products
Browse additional antibodies and detection reagents in the BioHippo catalog.
Detection uses the streptavidin system: pair with streptavidin-HRP for colorimetric or chemiluminescent detection (ELISA, WB), streptavidin-PE or streptavidin-FITC for flow cytometry, or streptavidin-AP for alkaline phosphatase-based IHC. No anti-Mouse secondary antibody is needed.
Endogenous biotin is present in liver, kidney, brain, and adipose tissue. Pre-treat tissue sections with an Avidin/Biotin Blocking Kit before applying this biotinylated antibody to suppress background from endogenous biotin.
Validated for IHC, ELISA, and Flow Cytometry. Working dilutions must be determined empirically for each application, sample type, and detection system.
No secondary antibody is required. Detection is achieved through streptavidin conjugated to your preferred enzyme or fluorophore, added after the antibody incubation step.
Store at 2–8°C. Stable for up to 1 year from receipt. Do not freeze — formulated in tissue culture supernatant; freeze-thaw may cause activity loss.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
