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Product Overview
Secondary Antibody
This mouse monoclonal antibody (Clone Strep-10, IgG1) targets streptavidin and tag proteins. Supplied unconjugated, it is detected via anti-Mouse secondary antibodies. Pre-validated at 1:20 for IHC, reducing optimization burden. Can be used to detect streptavidin-labeled molecules or tag proteins in indirect detection systems.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Immunohistochemistry | 1:20 |
| ELISA | Optimize experimentally |
Antibody Specifications
| Host Species | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone | Strep-10 |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Reactivity | Streptavidin / Tag proteins |
| Concentration | 200 µg/mL |
| Formulation | Supernatant with 1% BSA, 0.1% sodium azide |
| Storage | 2–8°C, up to 1 year |
| Mfr Catalogue No. | STJ16100613 |
Immunogen & Specificity
Immunogen: Streptavidin and tag protein antigen (proprietary).
Specificity: Reacts with streptavidin and tag proteins. Clone Strep-10.
Reactivity
Streptavidin/tag-specific; species-independent.
Safety & Handling
Research Use Only (RUO). Not for diagnostic or therapeutic applications. Formulation contains 1% BSA and 0.1% sodium azide — avoid ingestion. RUO only.
Related Products
Browse additional antibodies and detection reagents in the BioHippo catalog.
The recommended starting dilution for IHC is 1:20, as pre-validated by the manufacturer. Optimal dilution may vary based on tissue type, fixation method, antigen retrieval, and detection system — adjust empirically if needed.
Since this antibody is unconjugated, pair it with an anti-Mouse IgG secondary antibody appropriate for your detection method (e.g., anti-Mouse IgG-HRP for chromogenic IHC/ELISA, anti-Mouse IgG-FITC or Alexa Fluor for fluorescent IF).
Yes. This anti-Streptavidin antibody can detect streptavidin-conjugated molecules (streptavidin-HRP, streptavidin-PE, etc.) and tag proteins. Useful as a control reagent to validate streptavidin-based detection systems.
Store at 2–8°C upon receipt. Stable for up to 1 year. Do not freeze — BSA-containing formulations can aggregate upon freeze-thaw, leading to precipitation and activity loss.
Validated for IHC at 1:20 dilution. Performance may vary across tissue types, fixation protocols, and antigen retrieval conditions. Include a no-primary-antibody negative control to assess non-specific background in your tissue.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
