| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cystatin-C;Cystatin-3;Cst3; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: M1-A140 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma, Urine |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse cystatin-C (gene Cst3) in cell culture supernatants, serum, plasma ( heparin, EDTA) and urine. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Cystatin C (CST3) is a small secreted inhibitor of cysteine proteases, including cathepsins, made by nearly all nucleated cells. Because it is produced at a steady rate and cleared by glomerular filtration, its level in blood is widely used to estimate kidney function, and it is also studied in vascular and neurodegenerative disease.
Kit Components
| Anti-Mouse Cst3 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse Cst3 Standard | 2 vials, 20 ng/tube |
| Mouse Cst3 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, serum, plasma ( heparin, EDTA) and urine |
| Assay range | 312 pg/mL–20,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant mouse Cst3 (Met1–Ala140), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.2–7.2% (3 samples, n = 16 each) |
| Inter-assay CV | 4.7–8.3% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:200. In the supplier’s tests, serum and plasma samples measured about 800 ng/mL.
Example standard curve (OD450, ng/mL): 0 = 0.06; 0.312 = 0.1; 0.62 = 0.137; 1.25 = 0.202; 2.5 = 0.353; 5 = 0.625; 10 = 1.119; 20 = 1.923. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (ng/ml) | 0 | 0.312 | 0.62 | 1.25 | 2.5 | 5 | 10 | 20 |
| O.D. | 0.06 | 0.1 | 0.137 | 0.202 | 0.353 | 0.625 | 1.119 | 1.923 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 437 | 2310 | 9815 | 464 | 2268 | 10497 |
| Standard deviation | 27.96 | 97.02 | 706.68 | 34.8 | 106.59 | 871.25 |
| CV (%) | 6.4% | 4.2% | 7.2% | 7.5% | 4.7% | 8.3% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Zhu Xue-Xue et al. (2025) Inhibition of PGK1 ameliorates acute kidney injury through inactivating the PKM2/ALOX12/ferroptosis pathway in a study with male mice. Nature Communications. 10.1038/s41467-025-64480-1
Yongqiang Zhang et al. (2024) Polysaccharides from Dendrobium officinale delay diabetic kidney disease interstitial fibrosis through LncRNA XIST/TGF-β1. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2024.116636
Min-Xuan Xu et al. (2018) Activated iRhom2 drives prolonged PM2.5 exposure-triggered renal injury in Nrf2-defective mice. Nanotoxicology. 10.1080/17435390.2018.1513093
Xue-Xue Zhu et al. (2024) Cichoric acid ameliorates sepsis-induced acute kidney injury by inhibiting M1 macrophage polarization. European Journal of Pharmacology. 10.1016/j.ejphar.2024.176696
Wei Miaomiao et al. (2023) Notoginsenoside Fc ameliorates renal tubular injury and mitochondrial damage in acetaminophen-induced acute kidney injury partly by regulating SIRT3/SOD2 pathway. Frontiers in Medicine. 10.3389/fmed.2022.1055252
