| Field | Specification |
|---|---|
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Recombinant Mouse IL-2-RA/CD25 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu22-Lys236) of mouse CD25/IL-2Ralpha (Accession #NP_032393.3) fused with a hFc tag at the C-terminus. |
| Product Type | |
| Purity | |
| Storage | |
| UniProt # |
Product Overview
Research-grade Peptide Reagent: Mouse IL-2-RA/CD25 protein (Recombinant) (C-hFc) (STJP001474).
Target & Specificity
| NCBI Gene ID | 16184 |
|---|---|
| UniProt ID | P01590 |
| Immunogen | Recombinant Mouse IL-2-RA/CD25 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu22-Lys236) of mouse CD25/IL-2Ralpha (Accession #NP_032393.3) fused with a hFc tag at the C-terminus. |
| Immunogen Region (aa) | Glu22-Lys236 |
| Antibody Production Host | HEK293 cells |
| Conjugate | Unconjugated |
This peptide is designed for use specifically with the paired STJ-series antibody against the same target epitope. Cross-reactivity with other targets has not been evaluated and should be determined empirically.
Specifications
| Purity | > 95% by SDS-PAGE. |
|---|---|
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Validated Applications | Western Blot|Immunodepletion |
Recommended Use
For WB: load 5–20 ng per lane alongside experimental samples to confirm antibody function and expected band size. For IHC: apply to a positive-control tissue section at the concentration recommended for the paired antibody.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendorSt John's Laboratory LtdCatalog No.STJP001474UniProtP01590
Load 5–20 ng per WB lane alongside experimental samples to confirm anti-Mouse IL-2-RA/CD25 p antibody performance. For IHC, apply to a positive-control tissue section alongside experimental sections. Pair with antibody STJP001474 for matched validation.
For WB: 5–20 ng per lane produces a clear band under standard conditions. For IHC: use the concentration specified on the antibody datasheet. If no signal: confirm antibody is within expiry and antigen retrieval conditions are optimal.
This peptide is designed to pair with antibody STJP001474. Immunogen region: Recombinant Mouse IL-2-RA/CD25 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu22-Lys236) of . Using with a different antibody clone may not produce blocking results if epitopes differ.
Validated for Western Blot, Immunodepletion specificity controls. Performance in other applications (ELISA, IP) requires independent validation.
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.. Purity: >95% by HPLC. Prepare single-use aliquots to avoid freeze-thaw; stable ≥12 months at −20 °C when lyophilized.
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