| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | GIF; GLIF; MMIF; macrophage migration inhibitory factor|phenylpyruvate tautomerase |
| Product Type | |
| Sensitivity | |
| UniProt # |
Assay Principle
Sandwich ELISA
This kit employs a two-site sandwich ELISA to quantitate MIF in mouse samples. The microplate is pre-coated with anti-MIF capture antibody. Samples and standards bind the capture antibody; biotinylated anti-MIF detection antibody and Streptavidin-HRP are applied; TMB substrate generates colorimetric signal at 450 nm proportional to MIF concentration. Sensitivity: 31.25 pg/mL; range: 62.5–4,000 pg/mL.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Species Reactivity | Mouse (Mus musculus) |
| Target Analyte | MIF (Macrophage Migration Inhibitory Factor) |
| UniProt ID | P34884 |
| Sensitivity (LLOD) | 31.25 pg/mL |
| Detection Range | 62.5–4,000 pg/mL |
| Sample Types | ⚠ Not specified — confirm with manufacturer before use |
| Plate Format | 96-well, available as 48T or 96T |
| Kit Stability | ≤5% activity loss within stated shelf life |
| Manual | Available (PDF, see Documents tab) |
Standard Kit Components
| Component | Description |
|---|---|
| Microplate | 96-well pre-coated with capture antibody (12×8-well strips) |
| Standards | Recombinant MIF protein for standard curve |
| Detection Antibody | Biotinylated anti-MIF detection antibody |
| Streptavidin-HRP | Enzyme conjugate for signal amplification |
| TMB Substrate | 3,3′,5,5′-Tetramethylbenzidine chromogenic substrate |
| Stop Solution | Sulfuric acid stop solution |
| Wash Buffer | Concentrated wash buffer (dilute before use) |
| Sample Diluent | For sample preparation and dilution |
Scientific Background
Macrophage Migration Inhibitory Factor (MIF, UniProt P34884) is a pleiotropic pro-inflammatory cytokine originally identified as a T-lymphocyte product that inhibits random macrophage migration. MIF is constitutively expressed at high levels in macrophages, T cells, epithelial cells, and endocrine tissues. Unlike most cytokines, MIF counter-regulates glucocorticoid-mediated immunosuppression, enabling sustained inflammatory responses. MIF signals through the CD74/CD44 receptor complex, activating ERK1/2, PI3K/Akt, and NF-κB pathways. It is implicated in sepsis, rheumatoid arthritis, atherosclerosis, and cancer progression. Alternative names: GIF (glycosylation-inhibiting factor), GLIF, MMIF, phenylpyruvate tautomerase.
Safety & Handling
Research Use Only (RUO). Not for diagnostic or therapeutic applications. TMB substrate and stop solution are corrosive — wear appropriate PPE. Sodium azide may be present — avoid ingestion.
Related Products
Browse additional ELISA kits and immunoassay reagents in the BioHippo catalog.
MIF (Macrophage Migration Inhibitory Factor, UniProt P34884) is a pleiotropic pro-inflammatory cytokine that promotes macrophage activation and innate immune responses. Uniquely, MIF counter-regulates glucocorticoid-mediated immunosuppression, enabling sustained inflammation. MIF signals through CD74/CD44 and activates ERK1/2, PI3K/Akt, and NF-κB. It is implicated in sepsis, rheumatoid arthritis, atherosclerosis, and cancer progression.
Mouse serum MIF under basal conditions is typically 500–4,000 pg/mL — constitutively high compared to classical cytokines. LPS-stimulated macrophage supernatants may reach ng/mL concentrations. Pre-dilute serum samples 5–20-fold to bring concentrations within the standard curve range (62.5–4,000 pg/mL).
Not explicitly listed — consult the product manual (Documents tab) for validated matrices. Typical mouse MIF ELISA applications use serum, EDTA-plasma, or heparin-plasma, and cell culture supernatant. Avoid repeated freeze-thaw of stored samples.
MIF-2 (D-dopachrome tautomerase, D-DT) is a structurally homologous MIF family member sharing CD74 receptor signaling. This assay is designed to quantitate MIF (P34884 — the founding family member). Confirm MIF/MIF-2 cross-reactivity data with the manufacturer if your experiment requires distinguishing between these homologs.
Prepare an 8-point standard curve by 2-fold serial dilution of the provided Mouse MIF recombinant standard from 4,000 pg/mL to 62.5 pg/mL, plus a zero standard. Run in duplicate. Measure absorbance at 450 nm (reference 570 nm). Fit with a 4-parameter logistic (4PL) model. Multiply interpolated concentration by the sample dilution factor.
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