| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | SPNS1; FLJ38358; HSpin1; LAT; PP2030; SPIN1; SPINL; nrs; spinster homolog 1; spinster-like |
| Assay Time | |
| Assay Type | |
| Detection Method | |
| Gene ID | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Shipping | |
| Storage |
Features & Benefits
Mouse Protein spinster homolog 1 (SPNS1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPNS1. No significant cross-reactivity or interference between Mouse SPNS1 and analogues was observed.
Background
SPIN1 played a critical role in necrotic cell death in cultured human cells. SPIN1 bound the antiapoptotic protein BCL2 and the apoptosis regulator BCLXL and induced cell death via a pathway that was independent of mitochondrial cytochrome c (CYCS) release and caspase activation. Mutation analysis showed that SPIN1 bound BCLXL via its BH3 domain. Necrosis inhibitors, but not caspase inhibitors, blocked SPIN1-induced cell death. SPIN1-induced cell death increased autophagic vacuole formation and the mature form of lysosomal cathepsin D (CTSD), suggesting SPIN1 induces necrotic cell death via autophagy.SPIN1 expression was also detected in HeLa and HEK293 cell lines. SPIN1 localized diffusely throughout the cytoplasm and concentrated in a punctate pattern that colocalized with a mitochondrial marker.
This Protein spinster homolog 1 (SPNS1) ELISA kit is validated for use with Cell culture supernatants, Serum, Plasma, Other biological fluids. Samples should be collected, processed, and stored correctly to preserve analyte integrity — avoid repeated freeze-thaw cycles and centrifuge to remove particulates before use. Dilute samples exceeding the kit's detection range using the supplied assay diluent. Hemolytic, icteric, or lipemic samples may affect assay performance and should be tested with caution.
This is a sandwich ELISA kit employing an HRP (horseradish peroxidase)-conjugated secondary antibody paired with a TMB (3,3′,5,5′-tetramethylbenzidine) colorimetric substrate. In the sandwich format, the target analyte Protein spinster homolog 1 (SPNS1) captured on the microplate surface is detected by the conjugated antibody, generating a colorimetric signal proportional to analyte concentration. The reaction is stopped and absorbance measured at 450 nm on a standard microplate reader.
The complete protocol, from sample addition to final plate reading, requires approximately 3–5 hours. This includes two incubation periods (analyte binding and detection antibody steps), intermediate wash cycles to remove unbound material, 15–30 minutes of TMB substrate development, and final stop-solution addition before absorbance reading. Exact timing will vary with experience level and the number of samples processed in parallel.
Required equipment: (1) a microplate spectrophotometer capable of reading absorbance at 450 nm (reference wavelength 540–570 nm recommended for background correction); (2) precision single-channel or multichannel pipettes; (3) a plate washer or multichannel aspirator; (4) a microcentrifuge for sample clarification; and (5) a 37°C incubator or stable room-temperature environment. No fluorescence or luminescence reader is required — standard colorimetric plate readers are fully compatible with this kit.
This ELISA kit is formally validated for Mouse. Cross-reactivity with species not listed in the specification has not been independently characterized. Variability in protein sequence homology across species means that performance in unlisted species cannot be guaranteed without additional validation. For cross-species detection requirements or non-standard sample matrices, please contact BioHippo support or refer to the manufacturer's technical team for guidance.
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