Mouse Regulator of G-protein signaling 5 (RGS5) ELISA Kit

What it means: Sandwich ELISA is an immunoassay format that uses two antibodies — a capture antibody pre-coated on the microplate and a detection antibody — that bind simultaneously to different epitopes of the target analyte.

Why it matters:

• The sandwich format delivers high specificity and sensitivity by requiring the analyte to bind two independent antibodies simultaneously, dramatically reducing cross-reactivity with interfering matrix components.
• This format is broadly applicable across serum, plasma, cell culture supernatant, and tissue lysate matrices — the gold standard for quantitative protein detection in research.

How to interpret here: As a Sandwich ELISA kit, prepare fresh standards with each run, verify standard curve fit (R² ≥ 0.99), and use appropriate sample dilutions before interpolating concentrations.

What it means: The detection range 0.156-10 ng/mL defines the span of analyte concentrations within which this kit produces a quantitative, linear signal suitable for measurement.

Why it matters:

• Samples must fall within this range for reliable quantification. Concentrations above the upper limit require dilution; concentrations below the lower limit may need sample concentration or a more sensitive assay format.
• Run your standard curve in duplicate with each plate and verify linearity before interpolating unknown sample concentrations from the curve.

How to interpret here: Ensure your samples, after any required dilution, fall within 0.156-10 ng/mL. Perform a preliminary dilution series when working with novel sample types to identify the optimal dilution factor for accurate results.

What it means: Sandwich ELISA is an immunoassay format that uses two antibodies — a capture antibody pre-coated on the microplate and a detection antibody — that bind simultaneously to different epitopes of the target analyte.

Why it matters:

• The sandwich format delivers high specificity and sensitivity by requiring the analyte to bind two independent antibodies simultaneously, dramatically reducing cross-reactivity with interfering matrix components.
• This format is broadly applicable across serum, plasma, cell culture supernatant, and tissue lysate matrices — the gold standard for quantitative protein detection in research.

How to interpret here: As a Sandwich ELISA kit, prepare fresh standards with each run, verify standard curve fit (R² ≥ 0.99), and use appropriate sample dilutions before interpolating concentrations.