| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived mouse MPEG1 recombinant protein (Position: F31-D645) was used as the immunogen for the MPEG1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MPEG1 Antibody / Macrophage-expressed gene 1 / Perforin 2 is a anti-MPEG1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MPEG1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
MPEG1 is encoded by the MPEG1 gene on human chromosome 11q12.1. The protein contains a signal peptide, an N-terminal MACPF domain responsible for membrane pore formation, and a C-terminal transmembrane region that anchors it to endosomal and plasma membranes. MPEG1 is predominantly expressed in macrophages, dendritic cells, and other phagocytes, where it localizes to endolysosomal compartments. Upon bacterial invasion, MPEG1 is upregulated and translocated to pathogen-containing vacuoles, where it inserts into microbial membranes to mediate lysis.
The MPEG1 antibody reveals an ~79 kDa band in western blot corresponding to full-length MPEG1/Perforin-2. Immunofluorescence microscopy demonstrates punctate cytoplasmic and vesicular localization consistent with its endosomal distribution. Functional studies have shown that loss of MPEG1 impairs bacterial killing and increases susceptibility to infection. It acts downstream of pattern-recognition receptor activation, integrating with pathways controlled by NF-?B and interferon signaling. MPEG1 also interacts with ESCRT-associated and autophagic machinery to remove damaged or infected vacuoles.
In addition to antimicrobial defense, MPEG1 contributes to tissue homeostasis and inflammation control. It modulates cytokine production and can influence antigen presentation by professional phagocytes. Structural analyses reveal that MPEG1 forms ring-like oligomers that perforate target membranes, similar to complement MAC pores. The protein's activity is tightly regulated to prevent host cell damage.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
