| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MPLKIP recombinant protein (Position: E135-C179) was used as the immunogen for the MPLKIP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MPLKIP Antibody / M phase specific PLK1 interacting protein is a anti-MPLKIP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: MPLKIP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, ELISA
Biological background
The protein localizes to the nucleus and perinuclear regions, where it interfaces with chromatin and cell cycle machinery. Loss of function mutations in the MPLKIP gene, historically known as TTDN1, cause trichothiodystrophy non photosensitive type 1, a disorder characterized by brittle hair and developmental features, underscoring its role in nuclear homeostasis. Experimental systems suggest that M phase specific PLK1 interacting protein acts as a scaffold that positions kinases and effectors to ensure accurate phosphorylation timing, with downstream consequences for cohesion, condensation, and checkpoint signaling.
In the laboratory, researchers use MPLKIP antibody for immunoblotting to monitor expression across the cell cycle, for immunofluorescence to visualize nuclear patterns in interphase and mitosis, and for co-immunoprecipitation to examine complexes with PLK1 and associated regulators. When paired with synchronization, kinase inhibitors, or auxin-degron depletion, the antibody supports dissection of cause-and-effect relationships between protein abundance, phosphorylation events, and mitotic fidelity. In disease models, MPLKIP antibody enables evaluation of variant impact on stability and localization, clarifying genotype-phenotype mechanisms relevant to neurodevelopment and ectodermal biology.
The MPLKIP antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
