| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Myeloperoxidase was used as the immunogen for the MPO antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MPO Antibody / Myeloperoxidase is a anti-MPO Rabbit antibody Recombinant Rabbit Monoclonal clone 32M13 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MPO
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32M13, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
Myeloperoxidase has long been used as a histochemical marker for neutrophil activity. Detection with MPO antibody reveals the distribution and abundance of neutrophils in tissues, aiding in studies of infection, inflammation, and immune-mediated disorders. Elevated levels of myeloperoxidase are also associated with chronic inflammatory diseases, including atherosclerosis and rheumatoid arthritis. In cardiovascular research, plasma MPO concentration is studied as a potential biomarker for coronary artery disease and acute coronary syndromes.
Beyond host defense, excessive or misplaced MPO activity can cause tissue injury by generating reactive oxygen species and halogenated products. This has been implicated in neurodegenerative disorders such as Parkinson's and Alzheimer's disease. Using MPO antibody, researchers are able to map protein distribution in affected tissues and evaluate its contribution to disease progression.
In oncology, myeloperoxidase serves as a lineage marker distinguishing myeloid leukemias from lymphoid subtypes. Immunohistochemical detection with MPO antibody remains a diagnostic tool in hematopathology, where positive staining confirms myeloid origin. Flow cytometry and ELISA-based detection further expand applications of this antibody in clinical and research settings.
Because myeloperoxidase contributes to both protective immunity and pathological inflammation, it represents a dual-interest protein for immunologists, pathologists, and cardiovascular researchers.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
