| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human MSI2 was used as the immunogen for the MSI2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MSI2 Antibody / Musashi RNA-binding protein 2 is a anti-MSI2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MSI2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, FACS
Biological background
MSI2 is encoded by the MSI2 gene located on human chromosome 17q23.2. The protein is approximately 38 kilodaltons and contains two RNA recognition motifs (RRMs) at the N-terminus that specifically bind to target mRNAs containing UAG motifs in their 3' untranslated regions. Through this interaction, MSI2 represses translation of transcripts involved in differentiation while enhancing those promoting proliferation and stemness. MSI2 expression is particularly high in hematopoietic stem cells and neural progenitors.
The MSI2 antibody detects a 38 kilodalton band by western blot and shows cytoplasmic and perinuclear staining in stem cell populations and tumor cells. MSI2 regulates asymmetric cell division and maintains an undifferentiated state by antagonizing translational repressors such as PABP and interacting with the translational initiation machinery. In hematopoiesis, MSI2 cooperates with HOXA9 and MYC signaling to promote leukemic transformation. Overexpression correlates with poor prognosis in acute myeloid leukemia, breast cancer, and colorectal carcinoma.
In the nervous system, MSI2 modulates neurogenesis by controlling translation of key developmental regulators such as NUMB and REST. Loss of MSI2 leads to premature differentiation and reduced progenitor proliferation. Its function is tightly regulated by phosphorylation and interaction with signaling pathways including Notch and mTOR.
Because of its dual role in maintaining stemness and driving oncogenesis, MSI2 serves as a key marker and therapeutic target in cancer stem cell biology.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
