| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MTCH2 recombinant protein (Position: M1-I303) was used as the immunogen for the MTCH2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MTCH2 Antibody / Mitochondrial carrier homolog 2 is a anti-MTCH2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Rat. Reported localization: Cytoplasm (Mitochondria).
Key elements and design rationale
- Target: MTCH2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IP, IF, IHC, ICC, WB
Biological background
Functionally, MTCH2 antibody identifies a 305-amino-acid protein localized to the mitochondrial outer membrane. MTCH2 interacts with pro-apoptotic proteins such as BID and regulates mitochondrial outer membrane permeabilization (MOMP) during apoptosis. It modulates the recruitment and activation of truncated BID (tBID), a key step in BAX and BAK activation leading to cytochrome c release and caspase activation. Thus, MTCH2 serves as a checkpoint controlling apoptosis sensitivity.
The MTCH2 gene is located on chromosome 11p11.2 and encodes a protein expressed in metabolically active tissues including brain, liver, and skeletal muscle. MTCH2 contains six predicted transmembrane helices and shares limited homology with mitochondrial carrier family proteins, though it lacks classical solute transport function. Instead, it influences mitochondrial bioenergetics and lipid metabolism through protein-protein interactions at the outer membrane.
In addition to apoptosis, MTCH2 plays roles in energy metabolism and mitochondrial fission-fusion balance. It has been shown to interact with mitochondrial fission regulator DRP1 and influence cristae remodeling under metabolic stress. Genetic studies have associated MTCH2 variants with obesity and metabolic syndrome, suggesting that it contributes to energy balance by regulating mitochondrial efficiency and lipid oxidation.
In neuronal and immune systems, MTCH2 contributes to mitochondrial trafficking and immune cell activation. Overexpression of MTCH2 sensitizes cells to apoptotic stimuli, while its depletion can promote survival under stress conditions. Its dual role in metabolism and apoptosis underscores its function as a metabolic gatekeeper linking mitochondrial function to cell fate decisions.
MTCH2 antibody is widely used in mitochondrial biology, apoptosis, and metabolism research. It is suitable for western blotting, immunocytochemistry, and co-immunoprecipitation to study MTCH2 localization and interaction networks. This antibody supports studies of mitochondrial outer membrane regulation, lipid metabolism, and programmed cell death. In disease research, it assists in examining MTCH2's contribution to metabolic disorders and neurodegeneration.
Structurally, MTCH2 contains conserved hydrophobic transmembrane helices that form a scaffold for protein binding. It lacks the canonical carrier signature motifs found in other mitochondrial carriers, reflecting its specialized signaling function.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
