| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MTMR3 recombinant protein (Position: M1-E1074) was used as the immunogen for the MTMR3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MTMR3 Antibody / Myotubularin-related protein 3 is a anti-MTMR3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: MTMR3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS, ELISA
Biological background
Functionally, MTMR3 antibody identifies a 911-amino-acid cytoplasmic protein containing a PH-GRAM lipid-binding domain, a catalytic phosphatase domain, and a coiled-coil region responsible for dimerization. MTMR3 acts as a negative regulator of autophagy by dephosphorylating PI3P, thereby modulating membrane dynamics at autophagosome formation sites. It also regulates endosomal sorting and vesicle trafficking between endosomes and the trans-Golgi network.
The MTMR3 gene is located on chromosome 22q12.2 and is expressed in a broad range of tissues, including muscle, brain, and kidney. Its activity ensures proper membrane identity and turnover of signaling lipids within endocytic and autophagic pathways. Dysregulation of MTMR3 expression leads to aberrant phosphoinositide signaling, contributing to impaired autophagy, vesicle accumulation, and neurodegeneration.
In cellular signaling, MTMR3 integrates nutrient and stress signals to balance autophagy and cell survival. It interacts with mTORC1 and ULK1 complexes, coordinating lipid metabolism with autophagic flux. Loss or inhibition of MTMR3 enhances autophagy initiation, while overexpression suppresses it. In cancer, MTMR3 acts context-dependently, functioning as either a tumor suppressor or survival factor depending on cell type and metabolic state.
MTMR3 antibody is widely used in autophagy, membrane trafficking, and phosphoinositide signaling research. It is suitable for western blotting, immunofluorescence, and lipid signaling assays to detect MTMR3 expression and localization to endosomal membranes. In neurobiology, this antibody aids in studying lysosomal and endocytic dysfunction associated with neurodegenerative diseases. In cancer studies, MTMR3 detection contributes to understanding metabolic regulation and cell stress adaptation.
Structurally, MTMR3 contains a catalytic Cys-X5-Arg motif typical of the protein tyrosine phosphatase superfamily, with substrate specificity directed toward phosphatidylinositol derivatives. The PH-GRAM domain mediates lipid recognition, while the coiled-coil region enables dimer formation with other myotubularin family members.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
