| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human mTOR was used as the immunogen for the MTOR antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
mTOR Antibody / Mammalian target of rapamycin is a anti-mTOR Rabbit antibody Recombinant Rabbit Monoclonal clone 32M82 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: mTOR
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32M82, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
mTOR antibody is widely applied in cancer research, immunology, and metabolism. In cancer, hyperactivation of mTOR pathways drives uncontrolled proliferation and metabolic reprogramming. In immunology, mTOR regulates T cell differentiation and macrophage function. In metabolic biology, mTOR senses nutrient status and controls autophagy. By detecting mTOR protein, researchers can study how this kinase integrates diverse signals to maintain balance or promote disease.
Validated applications for mTOR antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blotting reveals mTOR expression and size, immunohistochemistry maps distribution in tumors and tissues, and immunofluorescence highlights subcellular localization at lysosomes and other compartments. These methods provide strong experimental tools to characterize mTOR biology.
Dysregulation of mTOR signaling contributes to cancer, diabetes, cardiovascular disease, and neurodegeneration. Mutations and upstream activation of PI3K and Akt lead to aberrant mTOR activity. Therapeutically, mTOR inhibitors such as rapamycin and its analogs are used to treat cancer and transplantation related immune suppression. Antibody based detection of mTOR supports both mechanistic studies and translational applications.
mTOR antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
