| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MVK recombinant protein (Position: K13-D394) was used as the immunogen for the MVK antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MVK Antibody / Mevalonate kinase is a anti-MVK Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: MVK
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, IP, FACS, ELISA
Biological background
MVK is encoded by the MVK gene located on human chromosome 12q24.11. The protein is approximately 396 amino acids long and localizes to the peroxisome and cytoplasm. MVK activity is regulated by feedback inhibition through cholesterol and isoprenoid intermediates, ensuring metabolic balance in lipid biosynthesis. The enzyme participates in the early steps of the mevalonate pathway, linking carbohydrate metabolism to lipid production.
The MVK antibody detects a 43 kilodalton protein by western blot and reveals diffuse cytoplasmic and peroxisomal staining under immunofluorescence microscopy. MVK provides precursors for essential biomolecules such as coenzyme Q, dolichol, and prenylated proteins, which are required for membrane integrity, protein glycosylation, and signal transduction.
Mutations in MVK cause mevalonic aciduria and hyperimmunoglobulinemia D syndrome, characterized by recurrent fever and inflammation due to impaired isoprenoid synthesis and excessive accumulation of mevalonic acid. MVK deficiency disrupts protein prenylation, mitochondrial function, and immune regulation, leading to autoinflammatory symptoms. The enzyme's activity is thus essential for cellular metabolism, immune homeostasis, and energy balance.
Because of its central role in cholesterol metabolism and inflammation, MVK is a crucial target for understanding metabolic regulation and disease pathogenesis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
