| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived mouse MYBBP1A recombinant protein (Position: D39-R1316) was used as the immunogen for the MYBBP1A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MYBBP1A Antibody / Myb-binding protein 1A is a anti-MYBBP1A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Mouse, Rat. Reported localization: Nucleus, Nucleolus, Cytoplasm.
Key elements and design rationale
- Target: MYBBP1A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, ELISA
Biological background
MYBBP1A plays a key role in ribosome biogenesis and nucleolar stress signaling. Upon cellular stress or energy depletion, MYBBP1A translocates from the nucleolus to the nucleoplasm, where it enhances p53 acetylation and activation, promoting cell cycle arrest and apoptosis. It also suppresses c-Myb-driven transcription to prevent uncontrolled proliferation. Through these mechanisms, MYBBP1A acts as a tumor suppressor and regulator of growth arrest. Depletion of MYBBP1A leads to impaired rRNA processing and increased genomic instability. Dysregulation of its expression has been reported in renal carcinoma, hepatocellular carcinoma, and hematologic malignancies.
The MYBBP1A antibody is used in cancer biology and transcriptional regulation studies to detect Myb-binding protein 1A localization and abundance. Western blot analysis typically identifies a ~150 kilodalton band, while immunofluorescence shows predominant nucleolar staining with redistribution upon stress. Because MYBBP1A interacts with both histone-modifying enzymes and transcriptional machinery, it is a critical factor for understanding nucleolar function and epigenetic regulation. The antibody is effective in chromatin immunoprecipitation, immunohistochemistry, and nuclear fractionation assays. The MYBBP1A antibody therefore supports research on tumor suppression, transcriptional control, and nucleolar biology.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
