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Description
One-step rapid mycoplasma detection kit (LAMP) (2G) is a rapid test for mycoplasma contamination in cell culture based on the LAMP method. The kit uses Yeasen's unique loop-mediated isothermal amplification (LAMP) technology to design specific primers for the conservative region of the mycoplasma 16S-23S rRNA sequence. It can directly use biological materials such as cell culture supernatant or serum as templates. If it is contaminated with mycoplasma, the conservative sequence of mycoplasma DNA will be amplified in large quantities and rapidly, turning the reaction solution from blue-purple to sky blue. The results can be distinguished by the naked eye without the need for electrophoresis.
This kit can detect 33 types of mycoplasma, including oral mycoplasma (M.orale), pneumoniae (M.pneumoniae), hyorhinis (M.hyorhinis), salivarium (M.salivarium), and arginini (M.arginini) required by domestic and foreign pharmacopoeia regulations. The sensitivity can reach 500 copies/uL. It can also amplify and react to 100 CFU/mL mycoplasma standard strains normally. It has the characteristics of strong specificity, high sensitivity, convenient and fast operation.
Compared with one-step rapid mycoplasma detection kit(1G), this product has been optimized and upgraded to reduce the false positive rate, improve the accuracy of the test results, and enhance the color recognition of negative and positive. After the amplification, the negative will not turn into positive after being placed at room temperature for a period of time, which does not affect the judgment of negative and positive.
Features
- Simple and rapid: One-step detection with easy-to-use protocol
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Broad compatibility: Directly applicable to culture supernatant, serum, and other sample types
Extensive coverage: Capable of detecting 33 mycoplasma species - High sensitivity: Detects as low as 500 copies/μL or 100 CFU/mL with a clear colorimetric change
- Safe & Contamination-Free: Contains UDG anti-contamination and non-infectious positive controls to ensure safety and eliminate the risk of cross-contamination
Specifications
|
For Use With (Equipment) |
Water bath or metal bath or PCR instrument |
|
Sample Type |
Strains, cell supernatants, serum etc. |
|
Polymerase |
Bst DNA Polymerase |
|
Detection Method |
LAMP |
|
Detection limit |
500 copies/μL |
|
Detection of mycoplasma types |
33 types, including 9 types of mycoplasma covered by the pharmacopoeia |
|
Reaction time (fast PCR procedure) |
30 min |
Components
|
Components No. |
Name |
40615ES08 |
40615ES25 |
40615ES60 |
|
40615-A |
MycAwayTM -Color LAMP Buffer |
85 μL |
425 μL |
425 μL×4 |
|
40615-B |
MycAwayTM -Color LAMP Enzyme |
5 uL |
25 μL |
25 μL×4 |
|
40615-C |
MycAwayTM -Color LAMP Primer |
10 μL |
50 μL |
50 μL×4 |
|
40615-D |
Mineral oil |
100 μL |
500 μL |
500 μL×4 |
|
40615-E |
Positive Control |
10 μL |
10 μL |
10 μL×4 |
Shipping and Storage
This product should be stored at -25~-15℃ for one year.
*If the entire kit is not used for a long time, please store it away from light.
Application
mycoplasma contamination; mycoplasma testing; Cell Culture Quality Control
Figures
1. Extensive coverage: Capable of detecting 33 mycoplasma species
2. Advanced detection capabilities
Figure 1. The MycAwayTM Plus-Color One-Step Mycoplasma Detection Kit (2G) provides robust detection performance than kits from other suppliers.
Using the same 100 CFU standard sample, 40615 reagent detects 100 CFU standard, which is not detectable by Supplier B*. Compared to Supplier A*, 40615 shows a more distinct color change with the same sample.
3. Superior inhibitor tolerance
Figure 2. Matrix tolerance of MycAwayTM Plus-Color One-Step Mycoplasma Detection Kit (2G)
4. Stability
Figure 3. Stability Test
(A) Freeze-thaw stability: The kit withstands 20 freeze-thaw cycles at -25 to -15°C without loss of performance.
(B) Heat-accelerated stability: The One-Step Mycoplasma Detection Kit (2G) remains stable after 14 days at 37°C.
Citations & References:
[1] Shi N, Yang Q, Zhang H, et al. Restoration of dystrophin expression in mice by suppressing a nonsense mutation through the incorporation of unnatural amino acids. Nat Biomed Eng. 2022;6(2):195-206. doi:10.1038/s41551-021-00774-1(IF:25.671)
[2] Zhang S, Yu F, Che A, et al. Neuroendocrine Regulation of Stress-Induced T Cell Dysfunction during Lung Cancer Immunosurveillance via the Kisspeptin/GPR54 Signaling Pathway. Adv Sci (Weinh). 2022;9(13):e2104132. doi:10.1002/advs.202104132(IF:16.806)
[3] Li Y, Xue B, Zhang M, et al. Transcription-coupled structural dynamics of topologically associating domains regulate replication origin efficiency. Genome Biol. 2021;22(1):206. Published 2021 Jul 12. doi:10.1186/s13059-021-02424-w(IF:13.583)
[4] Wu L, Xu Y, Zhao H, et al. FcγRIIB potentiates differentiation of myeloid-derived suppressor cells to mediate tumor immunoescape. Theranostics. 2022;12(2):842-858. Published 2022 Jan 1. doi:10.7150/thno.66575(IF:11.556)
[5] Tan B, Shi X, Zhang J, et al. Inhibition of Rspo-Lgr4 Facilitates Checkpoint Blockade Therapy by Switching Macrophage Polarization. Cancer Res. 2018;78(17):4929-4942. doi:10.1158/0008-5472.CAN-18-0152(IF:9.130)
[6] Yan G, Zhao H, Zhang Q, et al. A RIPK3-PGE2 Circuit Mediates Myeloid-Derived Suppressor Cell-Potentiated Colorectal Carcinogenesis. Cancer Res. 2018;78(19):5586-5599. doi:10.1158/0008-5472.CAN-17-3962(IF:9.130)
[7] Gu Z, Shi C, Li J, et al. Palbociclib-based high-throughput combination drug screening identifies synergistic therapeutic options in HPV-negative head and neck squamous cell carcinoma. BMC Med. 2022;20(1):175. Published 2022 May 12. doi:10.1186/s12916-022-02373-6(IF:8.775)
[8] Wu L, Zhang X, Zheng L, et al. RIPK3 Orchestrates Fatty Acid Metabolism in Tumor-Associated Macrophages and Hepatocarcinogenesis. Cancer Immunol Res. 2020;8(5):710-721. doi:10.1158/2326-6066.CIR-19-0261(IF:8.728)
[9] Qin J, Zhang X, Tan B, et al. Blocking P2X7-Mediated Macrophage Polarization Overcomes Treatment Resistance in Lung Cancer. Cancer Immunol Res. 2020;8(11):1426-1439. doi:10.1158/2326-6066.CIR-20-0123(IF:8.728)
[10] Cao M, Huang W, Chen Y, et al. Chronic restraint stress promotes the mobilization and recruitment of myeloid-derived suppressor cells through β-adrenergic-activated CXCL5-CXCR2-Erk signaling cascades. Int J Cancer. 2021;149(2):460-472. doi:10.1002/ijc.33552(IF:7.396)
Documents:
Safety Data Sheet
Manuals
40615_Manual_Ver.EN20250910.pdf
Mycoplasma contamination is one of the most prevalent and insidious threats in cell culture, with estimates suggesting 15–35% of cell lines are contaminated at any given time. Unlike bacterial contamination, mycoplasma does not cause visible turbidity. Contaminated cells exhibit altered metabolism, gene expression, proliferation rates, cytokine secretion, and receptor signaling — invalidating experimental results. Regular testing is mandatory before publication, biobanking, or use in drug discovery assays.
This kit targets the most common cell culture contaminants, including Mycoplasma hyorhinis, M. orale, M. fermentans, M. arginini, M. hominis, Acholeplasma laidlawii, and others. Universal primer or probe designs used in PCR/qPCR-based kits detect conserved mycoplasma-specific sequences and may detect up to 70+ species. Refer to the species coverage table in the product datasheet for validated strains.
Best practices recommend testing all incoming cell stocks, after every 2–4 weeks in continuous culture, before cryopreservation, before experiments generating data for publication, and after any culture manipulation (transduction, transfection, co-culture). For GMP and biopharmaceutical environments, testing frequency and documentation must comply with regulatory agency guidelines (ICH Q5A/EMA/FDA).
Protocol requirements vary by kit format. MycAway colorimetric kits use culture supernatant directly (no extraction required), providing results in approximately 2 hours. PCR/qPCR-based kits (GMyc-PCR, qPCR variants) may require a brief extraction or heat lysis step for optimal sensitivity. The qPCR format provides quantitative data and is preferred for regulatory submissions. Refer to the specific product datasheet for the validated input protocol.
Yeasen Biotechnology offers flexible customization options for many of its assay kits and detection reagents, including custom lot sizes, bulk ordering, and application-specific formulation adjustments. Volume pricing, custom packaging, and kit bundling may be available depending on the product and intended workflow. A Certificate of Analysis (CoA) and lot-specific QC data are provided with every order. For inquiries regarding large-volume orders, custom configurations, or integration into automated workflows, please contact the BioHippo team for a tailored quotation.
