| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | IL-2; T-cell growth factor; TCGF; Lymphokine; Aldesleukin (therapeutic form) |
| Biological Activity | |
| Endotoxin Level | |
| Expression System | |
| Form | Lyophilized powder |
| Formulation | |
| Gene ID | |
| Molecular Weight | |
| Post-Translational Modifications | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage | |
| Target |
IL-2 (Interleukin-2) is a pleiotropic T-cell growth factor and immunomodulatory cytokine that signals through the high-affinity IL-2 receptor complex (IL-2Rα/β/γ chain) to activate JAK/STAT5, PI3K/Akt, and MAPK pathways. It plays essential roles in T-cell proliferation, NK cell expansion, regulatory T-cell (Treg) maintenance, and broader lymphocyte homeostasis. As a non-glycosylated single-chain protein (~15.3 kDa), IL-2 activity critically depends on the native Cys58–Cys105 intramolecular disulfide bond.
Expressed in Pichia pastoris (K. phaffii)
MycoFold™ IL-2 is produced in a proprietary engineered yeast platform that ensures chaperone-guided protein folding, lysis-free secretion, and elimination of animal-origin inputs and bacterial endotoxin. The result is a highly pure, bioactive protein that reconstitutes in deionized water — no special buffers required.
Product Specifications
| Cat. No. | GR1004-10, GR1004-50, GR1004-100, GR1004-250, GR1004-500, GR1004-1000 |
|---|---|
| Source | Pichia pastoris (K. phaffii) |
| Structure / Form | Carrier protein-free; no affinity tags; non-glycosylated single-chain monomer |
| Molecular Weight | ~15.3 kDa (monomer; non-glycosylated single-chain) |
| UniProt / Gene | P60568 / IL2 (NCBI Gene ID: 3558) |
| Purity | ≥98% by Mass Spectrometry (mass spec confirmed) |
| Endotoxin | See lot-specific CoA (LAL tested per lot) |
| Storage | Lyophilized: ≤−20°C until expiry. Reconstituted: 2–8°C short-term; −20°C for extended storage. Avoid repeated freeze-thaw cycles. |
| Shipping | Ambient temperature; lyophilized formulation requires no cold chain |
QC & Validation
✓ Lot-specific CoA ✓ SDS-PAGE Purity ✓ LAL Endotoxin Tested ✓ Mycoplasma Tested (qPCR) ✓ BSE/TSE Statement AvailableSafety & Regulatory
| Cat. No. | GR1004-10, GR1004-50, GR1004-100, GR1004-250, GR1004-500, GR1004-1000 |
| Expression System | Pichia pastoris (K. phaffii), eukaryotic yeast |
| Protein Structure | Non-glycosylated single-chain monomer; Cys58–Cys105 intramolecular disulfide; lyophilized powder |
| Molecular Weight | ~15.3 kDa (monomer, non-glycosylated) |
| UniProt / Gene | P60568 / IL2 (NCBI Gene ID: 3558) |
| Species Reactivity | Human, Mouse (cross-reactive) |
| Specific Activity | See lot-specific CoA (T-cell proliferation assay) |
| Purity | ≥98% by mass spectrometry |
| Endotoxin | See lot-specific CoA (LAL tested per lot) |
| Additional QC Tests | Host cell protein (HCP), host cell DNA, mycoplasma (qPCR) |
| Formulation | Carrier protein-free; no affinity tags; no animal-origin excipients |
| Reconstitution | Deionized water, 0.3–0.5 mg/mL; no buffers required |
| Storage (Lyophilized) | ≤−20°C until expiry |
| Storage (Reconstituted) | 2–8°C short-term; −20°C extended storage; avoid repeated freeze-thaw |
| Shipping | Ambient temperature; no cold chain required |
Bioactivity: MycoFold rHu IL-2 specific activity is determined by T-cell proliferation assay (CTLL-2 or equivalent) per lot and reported in the lot-specific Certificate of Analysis. Chaperone-guided folding in the Pichia pastoris ER pathway generates the native Cys58–Cys105 intramolecular disulfide bond, which is essential for correct IL-2 receptor (IL-2Rα/β/γ complex) binding and downstream JAK/STAT5 signaling.
Purity Verification: Purity ≥98% confirmed by mass spectrometry on every lot — providing both identity confirmation and highest-confidence purity assessment. Additional QC includes host cell protein (HCP) by immunoassay, host cell DNA by qPCR, and mycoplasma by qPCR.
Structural Note: IL-2 is a non-glycosylated protein in its native form. The Pichia pastoris expression system is optimized for the production of non-glycosylated proteins with proper disulfide formation, yielding a homogeneous single-chain species at ~15.3 kDa with consistent lot-to-lot performance.
MycoFold™ rHu IL-2 supports a broad range of immunology and oncology research applications:
- T-Cell Expansion & Activation: CD4⁺ and CD8⁺ T-cell proliferation; regulatory T-cell (Treg) expansion; TIL and NK cell cultures
- Immunotherapy Research: CAR-T cell manufacturing protocols; IL-2 signal transduction studies (IL-2Rα/β/γ); cytokine dose-response experiments
- Cancer Immunology: Tumor-infiltrating lymphocyte (TIL) expansion; anti-tumor T-cell effector function assays; cancer immunotherapy co-culture models
- Cytokine Biology: JAK/STAT5 pathway activation studies; PI3K/Akt and MAPK downstream signaling; immune cell crosstalk models
- Hematopoiesis: Lymphocyte progenitor differentiation; NK cell development and expansion; innate lymphoid cell (ILC) culture
No. Human IL-2 is naturally a non-glycosylated protein. MycoFold rHu IL-2 is expressed in Pichia pastoris without glycosylation, consistent with the native human protein structure. The critical structural feature is the Cys58–Cys105 intramolecular disulfide bond, which is correctly formed via chaperone-guided folding in the yeast ER, yielding a properly folded single-chain monomer at ~15.3 kDa.
Specific activity is determined by T-cell proliferation assay using CTLL-2 cells or an equivalent validated T-cell proliferation system. Each lot is tested and the specific activity value (IU/mg) is reported in the lot-specific Certificate of Analysis (CoA). Additional QC tests per lot include purity by mass spectrometry (≥98%), endotoxin by LAL, host cell protein (HCP), host cell DNA, and mycoplasma by qPCR.
Yes. MycoFold rHu IL-2 is suitable for T-cell expansion protocols including CAR-T cell manufacturing workflows. Its carrier protein-free, animal-free formulation makes it compatible with defined and GMP-grade culture processes. Reconstitute in deionized water and add to culture medium per your established protocol. Lot-specific CoA documentation supports traceability requirements.
Typical IL-2 working concentrations for T-cell expansion range from 10–100 IU/mL (approximately 0.5–5 ng/mL equivalent, depending on specific activity). Optimal concentrations depend on your cell type, culture system, and experimental endpoint. We recommend titrating IL-2 in your assay system and consulting the lot CoA for specific activity to calculate precise IU-based dosing. Contact our scientific support team for protocol guidance.
The following customization options may be available for qualified research programs:
- Custom Lot Sizes: Quantities >1 mg available for high-volume T-cell expansion or longitudinal studies
- Institutional Volume Pricing: Core lab and procurement programs available
- GMP-Compatible Supply: Animal-free manufacturing and full documentation (CoA, HCP, DNA, mycoplasma) support regulatory filing requirements
Contact our team to discuss your requirements.
