| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Myosin Light Chain 2/MLC-2V/MYL2 was used as the immunogen for the MYL2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MYL2 Antibody / Myosin regulatory light chain 2 is a anti-MYL2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Monkey, Mouse, Rat.
Key elements and design rationale
- Target: MYL2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB
Biological background
Functionally, MYL2 antibody identifies a calcium-binding protein that undergoes phosphorylation by myosin light chain kinase (MLCK) at serine 15. Phosphorylation increases calcium sensitivity and enhances contractile force, playing a vital role in excitation-contraction coupling. MYL2 expression is strongest in cardiac ventricles, distinguishing it from atrial isoforms such as MYL7. Its presence marks ventricular myocytes in both mature and differentiating cardiac tissue.
The MYL2 gene resides on chromosome 12q24.11 and encodes a regulatory component of the myosin complex. Mutations in MYL2 have been associated with familial hypertrophic cardiomyopathy, left ventricular dysfunction, and sudden cardiac death. These variants alter protein folding or phosphorylation sites, impairing actomyosin cross-bridge cycling and contractile mechanics.
In muscle physiology, MYL2 acts as a fine-tuner of myosin motor function, adjusting contraction kinetics through dynamic phosphorylation. In developmental studies, MYL2 serves as a ventricular differentiation marker, while in pathology it reflects remodeling in cardiac hypertrophy and failure. Experimental models using MYL2 antibody provide insights into sarcomere assembly, cardiomyocyte maturation, and myofibrillar alignment.
MYL2 antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence to detect cardiac myosin light chain expression. It is extensively used in cardiovascular research, muscle biology, and regenerative medicine to monitor contractile protein regulation.
Structurally, MYL2 belongs to the EF-hand calcium-binding family and interacts with the IQ motif of myosin heavy chains. Its N-terminal domain modulates ATP hydrolysis and contributes to sarcomeric order. This antibody enables precise detection of MYL2 to study contractile regulation, disease mechanisms, and cardiomyocyte differentiation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
