| Field | Specification |
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| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human MYL3 was used as the immunogen for the MYL3 antibody. |
| Isotype | |
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| Purity | |
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Overview
MYL3 Antibody / Myosin light chain 3 is a anti-MYL3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MYL3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB
Biological background
Functionally, MYL3 antibody identifies a 151-amino-acid calcium-binding protein localized to the myofilament's thick filament region. MYL3 interacts with the myosin heavy chain alpha isoform and regulates actomyosin ATPase activity through conformational changes induced by calcium binding. It plays a key role in sarcomere assembly, contractile force regulation, and muscle fiber maintenance.
The MYL3 gene is located on chromosome 3p21.31 and is expressed predominantly in ventricular cardiac tissue and slow-twitch skeletal muscles. MYL3 is essential for normal heart contractility and mechanical stability under physiological stress.
Pathologically, mutations in MYL3 cause familial hypertrophic cardiomyopathy and restrictive cardiomyopathy by altering calcium binding and myosin-actin interactions. Defects in MYL3 impair contractile performance and promote myofibrillar disarray. Research using MYL3 antibody supports studies in cardiac physiology, muscle biochemistry, and hereditary myopathies.
MYL3 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect sarcomeric proteins.
Structurally, Myosin light chain 3 belongs to the EF-hand superfamily and contains two calcium-binding motifs that regulate its conformational changes and interaction with myosin. This antibody aids analysis of MYL3's role in cardiac contractility and muscle development.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
