| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequencein the middle region of human MYL4 was used as the immunogen for the MYL4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MYL4 Antibody / Myosin light chain 4 is a anti-MYL4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MYL4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB
Biological background
MYL4 is expressed abundantly in atrial cardiomyocytes, where it contributes to the high contraction frequency of the atria. During development, MYL4 is also expressed in ventricles and skeletal muscle before being downregulated postnatally, replaced by other myosin light chain isoforms. Re-expression of MYL4 in adult ventricles has been reported under pathological conditions such as hypertrophic cardiomyopathy, heart failure, and atrial fibrillation, suggesting a role in cardiac remodeling and disease adaptation. The protein forms part of the sarcomeric thick filament complex and interacts with myosin heavy chain alpha and beta isoforms to regulate actin-myosin cross-bridge cycling.
The MYL4 antibody is widely used in cardiovascular and muscle physiology research to study contractile protein composition, developmental regulation, and disease-associated remodeling. Western blot analysis typically identifies a 22 kilodalton band corresponding to MYL4, while immunohistochemistry reveals strong cytoplasmic staining in atrial tissue and developing muscle fibers. The antibody assists in assessing cardiac maturation, contractile diversity, and cellular hypertrophy mechanisms. Because MYL4 has been implicated in atrial fibrillation and abnormal calcium handling, its detection provides diagnostic and mechanistic insights into heart rhythm disorders.
Beyond the heart, MYL4 contributes to cytoskeletal organization and mechanotransduction in non-muscle cells. It also interacts with actin-regulating kinases and phosphatases that modify contractile responses. In molecular studies, MYL4 serves as a marker of atrial differentiation and a target for evaluating cardiac-specific gene expression in regenerative models. The MYL4 antibody supplied by
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
